Pandemic H1N1 influenza a viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies

E R Job; Y M Deng; M D Tate; B Bottazzi; E C Crouch; Melinda M Dean; A Mantovani; A G Brooks; P C Reading

doi:10.4049/jimmunol.1001613

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Pandemic H1N1 influenza a viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies

Journal article

Peer reviewed

Pandemic H1N1 influenza a viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies

E R Job, Y M Deng, M D Tate, B Bottazzi, E C Crouch, Melinda M Dean, A Mantovani, A G Brooks and P C Reading

Journal of Immunology, Vol.185(7), pp.4284-4291

2010

DOI: https://doi.org/10.4049/jimmunol.1001613

PMID: 20817882

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Abstract

collectin

mannose binding lectin

pentraxin

surfactant protein D

virus sialidase

C reactive protein

hemagglutinin

pentraxin 3

serum amyloid P

sialidase

animal cell

antiviral activity

article

controlled study

enzyme activity

female

glycosyla

Acquired immune responses elicited to recent strains of seasonal H1N1 influenza viruses provide limited protection against emerging A(H1N1) pandemic viruses. Accordingly, pre-existing or rapidly induced innate immune defenses are of critical importance in limiting early infection. Respiratory secretions contain proteins of the innate immune system, including members of the collectin and pentraxin superfamilies. These mediate potent antiviral activity and act as an initial barrier to influenza infection. In this study, we have examined the sensitivity of H1N1 viruses, including pandemic virus strains, for their sensitivity to collectins (surfactant protein [SP]-D and mannose-binding lectin [MBL]) and to the pentraxin PTX3. Human SP-D and MBL inhibited virus-induced hemagglutinating activity, blocked the enzymatic activity of the viral neuraminidase, and neutralized the ability of H1N1 viruses to infect human respiratory epithelial cells in a manner that correlated with the degree of glycosylation in the globular head of the hemagglutinin. Recent seasonal H1N1 viruses expressed three to four N-glycosylation sequons on the head of hemagglutinin and were very sensitive to inhibition by SP-D or MBL, whereas A(H1N1) pandemic viruses expressed a single N-glycosylation sequon and were resistant to either collectin. Of interest, both seasonal and pandemic H1N1 viruses were resistant to PTX3. Thus, unlike recent seasonal H1N1 strains of influenza virus, A(H1N1) pandemic viruses are resistant to the antiviral activities of innate immune proteins of the collectin superfamily.

Details

Title: Pandemic H1N1 influenza a viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies
Authors: E R Job (Author) - The University of Melbourne
Y M Deng (Author) - World Health Organization Collaborating Centre for Reference and Research on Influenza
M D Tate (Author) - The University of Melbourne
B Bottazzi (Author) - IRCCS Humanitas Research Hospital
E C Crouch (Author) - Washington University in St. Louis
Melinda M Dean (Author) - Australian Red Cross Blood Service
A Mantovani (Author) - University of Milan
A G Brooks (Author) - The University of Melbourne
P C Reading (Author) - The University of Melbourne
Publication details: Journal of Immunology, Vol.185(7), pp.4284-4291
Publisher: American Association of Immunologists
Date published: 2010
DOI: 10.4049/jimmunol.1001613
ISSN: 0022-1767; 1550-6606; 0022-1767
PMID: 20817882
Organisation Unit: School of Health - Biomedicine; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99513782602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology

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