Packed Red Blood Cell Transfusion Modulates Myeloid Dendritic Cell Activation and Inflammatory Response in Vitro

K K Ki; Helen M Faddy; R L Flower; Melinda M Dean

doi:10.1089/jir.2017.0099

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Packed Red Blood Cell Transfusion Modulates Myeloid Dendritic Cell Activation and Inflammatory Response in Vitro

Journal article

Peer reviewed

Packed Red Blood Cell Transfusion Modulates Myeloid Dendritic Cell Activation and Inflammatory Response in Vitro

K K Ki, Helen M Faddy, R L Flower and Melinda M Dean

Journal of Interferon & Cytokine Research, Vol.38(3), pp.111-121

2018

DOI: https://doi.org/10.1089/jir.2017.0099

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Abstract

blood dendritic cell antigen 3

immune modulation

myeloid dendritic cells

packed red blood cells

transfusion

Transfusion of packed red blood cells (PRBCs) modulates patients' immune responses and clinical outcomes; however, the underpinning mechanism(s) remain unknown. The potential for PRBC to modulate myeloid dendritic cells (mDC) and blood DC antigen 3 was assessed using an in vitro transfusion model. In parallel, to model processes activated by viral or bacterial infection, toll-like receptor agonists polyinosinic:polycytidylic acid or lipopolysaccharide were added. Exposure to PRBC upregulated expression of CD83 and downregulated CD40 and CD80 on both DC subsets, and it suppressed production of interleukin (IL)-6, IL-8, IL-12, tumor necrosis factor-α, and interferon-gamma-inducible protein-10 by these cells. Similar effects were observed when modeling processes activated by concurrent infection. Furthermore, exposure to PRBC at date of expiry was associated with more pronounced effects in all assays. Our study suggests PRBC have an impact on recipient DC function, which may result in failure to establish an appropriate immune response, particularly in patients with underlying infection.

Details

Title: Packed Red Blood Cell Transfusion Modulates Myeloid Dendritic Cell Activation and Inflammatory Response in Vitro
Authors: K K Ki (Corresponding Author) - Australian Red Cross Blood Service
Helen M Faddy (Author) - Australian Red Cross Blood Service
R L Flower (Author) - Australian Red Cross Blood Service
Melinda M Dean (Author) - Australian Red Cross Blood Service
Publication details: Journal of Interferon & Cytokine Research, Vol.38(3), pp.111-121
Publisher: Mary Ann Liebert, Inc. Publishers
Date published: 2018
DOI: 10.1089/jir.2017.0099
ISSN: 1079-9907
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450989702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology; Immunology

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