Journal article
PCR detection and differentiation of Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia trachomatis
Molecular and Cellular Probes, Vol.6(5), pp.389-394
1992
Abstract
A PCR-based system was developed for the detection and differentiation of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae. A conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. The three species were then differentiated from each other by digestion of this PCR product with restriction enzymes Eco RI and either Hin dlll or Pst l. The system was shown to work for two strains of C. pneumoniae, 11 strains of C. psittaci and 10 serovars of C. trachomatis, and had a sensitivity of less than 10 chlamydial elementary bodies. This method was also applicable to the detection of C. trachomatis in conjunctival and nasopharyngeal swabs. © 1992.
Details
- Title
- PCR detection and differentiation of Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia trachomatis
- Authors
- S J Rasmussen (Author) - Queensland University of TechnologyF P Douglas (Author) - Menzies School of Health ResearchPeter Timms (Author) - Queensland University of Technology
- Publication details
- Molecular and Cellular Probes, Vol.6(5), pp.389-394
- Publisher
- Academic Press
- Date published
- 1992
- DOI
- 10.1016/0890-8508(92)90032-S
- ISSN
- 0890-8508
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449846802621
- Output Type
- Journal article
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