PCR-Based Assay to Survey for Knockdown Resistance to Pyrethroid Acaricides in Human Scabies Mites (Sarcoptes Scabiei Var Hominis)

C Pasay; Shelley F Walton; K Fischer; D C Holt; J McCarthy

doi:10.4269/ajtmh.2006.74.649

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PCR-Based Assay to Survey for Knockdown Resistance to Pyrethroid Acaricides in Human Scabies Mites (Sarcoptes Scabiei Var Hominis)

Journal article

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Peer reviewed

PCR-Based Assay to Survey for Knockdown Resistance to Pyrethroid Acaricides in Human Scabies Mites (Sarcoptes Scabiei Var Hominis)

C Pasay, Shelley F Walton, K Fischer, D C Holt and J McCarthy

American Journal of Tropical Medicine and Hygiene, Vol.74(4), pp.649-657

2006

DOI: https://doi.org/10.4269/ajtmh.2006.74.649

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Abstract

Medical Microbiology

scabies

pyrethroid

knockdown resistance

Permethrin, in the form of a topical cream, is being increasingly used for community-based programs to control endemic scabies. The development of resistance has reduced the use of pyrethroids for the control of many arthropods of economic and health importance. The best recognized form of pyrethroid resistance, known as knockdown resistance or kdr, has been linked to specific mutations in the target of these agents, the para-homologous voltage-sensitive sodium channel gene (Vssc). To develop tools to study resistance to pyrethroid acaricides, we cloned 3711 and 6151 bp, respectively, of cDNA and genomic fragments of the Vssc gene from scabies mite, Sarcoptes scabiei. The sequence encompasses the major polymorphic amino acid residues associated with pyrethroid resistance. A polymerase chain reaction-based strategy has been developed that enables genotyping individual scabies mites. This will facilitate early detection and monitoring of pyrethroid resistance in scabies mite populations under drug selection pressure.

Details

Title: PCR-Based Assay to Survey for Knockdown Resistance to Pyrethroid Acaricides in Human Scabies Mites (Sarcoptes Scabiei Var Hominis)
Authors: C Pasay (Author) - Queensland Institute of Medical Research
Shelley F Walton (Author) - Charles Darwin University
K Fischer (Author) - Charles Darwin University
D C Holt (Author) - Charles Darwin University
J McCarthy (Author) - Charles Darwin University
Publication details: American Journal of Tropical Medicine and Hygiene, Vol.74(4), pp.649-657
Publisher: American Society of Tropical Medicine and Hygiene
Date published: 2006
DOI: 10.4269/ajtmh.2006.74.649
ISSN: 0002-9637
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
Language: English
Record Identifier: 99449682402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Public, Environmental & Occupational Health; Tropical Medicine

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