Orally Inoculated Eschericia coli Strains Colonize the Gut and Increase Bacterial Translocation After Stress in Rats

C Nettelbladt; Mohammad Katouli; T Bark; T Svenberg; R Mollby; O Ljungqvist

doi:10.1097/01.shk.0000068324.41183.c2

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Orally Inoculated Eschericia coli Strains Colonize the Gut and Increase Bacterial Translocation After Stress in Rats

Journal article

Peer reviewed

Orally Inoculated Eschericia coli Strains Colonize the Gut and Increase Bacterial Translocation After Stress in Rats

C Nettelbladt, Mohammad Katouli, T Bark, T Svenberg, R Mollby and O Ljungqvist

Shock, Vol.20(3), pp.251-256

2003

DOI: https://doi.org/10.1097/01.shk.0000068324.41183.c2

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Abstract

Clinical Sciences

Escherichia coli

translocating strains

biochemical fingerprinting

bacterial translocation

experimental hemorrhage

Coliforms are the members of the indigenous gut flora that most often translocate to mesenteric lymph nodes. Very few strains of Escherichia coli found in cecal contents of rats are able to translocate. The present study investigated the role of the composition of the gut flora for the occurrence of bacterial translocation. Two strains of E. coli (KI-C1 and KI-C2), previously shown to translocate in rats subjected to stress, were given by oral inoculation to rats lacking these strains. A biochemical fingerprinting method was used to identify bacteria in cecal contents, on cecal epithelium, and in mesenteric lymph nodes. In the inoculated group, starved for 24 h, seven out of 11 rats showed translocation, whereas in the noninoculated group one of 11 rats showed translocation. In groups subjected to hemorrhage and then starved for 24 h, 15/22 rats in the inoculated and 5/20 rats in the noninoculated group showed translocation. These findings show that orally inoculated KI-C1 and KI-C2 strains can colonize the gut and can substantially increase bacterial translocation in rats subjected to mild and severe stress. The composition of the gut flora seems to be an underestimated factor in the pathophysiology of bacterial translocation.

Details

Title: Orally Inoculated Eschericia coli Strains Colonize the Gut and Increase Bacterial Translocation After Stress in Rats
Authors: C Nettelbladt (Author) - Karolinska Institute, Sweden
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
T Bark (Author) - Karolinska Institute, Sweden
T Svenberg (Author) - Karolinska Institute, Sweden
R Mollby (Author) - Karolinska Institute, Sweden
O Ljungqvist (Author) - Karolinska Institute, Sweden
Publication details: Shock, Vol.20(3), pp.251-256
Publisher: Lippincott Williams & Wilkins
Date published: 2003
DOI: 10.1097/01.shk.0000068324.41183.c2
ISSN: 1073-2322
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449006402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Critical Care Medicine; Hematology; Peripheral Vascular Disease; Surgery

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