Journal article
Optimization of liver glycogen extraction when considering the fine molecular structure
Carbohydrate Polymers, Vol.261, pp.1-11
2021
PMID: 33766374
Abstract
Liver glycogen is a branched glucose polymer that functions as a blood-sugar buffer in animals. Previous studies have shown that glycogen’s molecular structure affects its properties. This makes it important to develop a technique that extracts and purifies a representative sample of glycogen. Here we aim to optimize the sucrose density gradient centrifugation method for preserving glycogen’s molecular structure by varying the density of the sucrose solution. The preservation of glycogen’s structure involves: 1) minimizing molecular damage and 2) obtaining a structurally representative sample of glycogen. The addition of a 10-minute boiling step was also tested as a means for denaturing any glycogen degrading enzymes. Lower sucrose concentrations and the introduction of the boiling step were shown to be beneficial in obtaining a more structurally representative sample, with the preservation of smaller glycogen particles and decreased glycogen chain degradation.
Details
- Title
- Optimization of liver glycogen extraction when considering the fine molecular structure
- Authors
- Ziyi Wang - University of QueenslandQinghua Liu - Xuzhou Medical CollegeLiang Wang - Xuzhou Medical CollegeRobert G. Gilbert (Corresponding Author) - Yangzhou UniversityMitchell A Sullivan (Corresponding Author) - Translational Research Institute
- Publication details
- Carbohydrate Polymers, Vol.261, pp.1-11
- Publisher
- Elsevier Ltd
- Date published
- 2021
- DOI
- 10.1016/j.carbpol.2021.117887
- ISSN
- 1879-1344
- PMID
- 33766374
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035094802621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Applied
- Chemistry, Organic
- Polymer Science
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