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Optimization of Culture of Leptospira from Humans with Leptospirosis
Journal article   Open access   Peer reviewed

Optimization of Culture of Leptospira from Humans with Leptospirosis

V Wuthiekanun, W Chierakul, D Limmathurotsakul, Lee D Smythe, M L Symonds, M F Dohnt, A T Slack, R Limpaiboon, Y Suputtamongkol, N J White, …
Journal of Clinical Microbiology, Vol.45(4), pp.1363-1365
2007
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https://doi.org/10.1128/JCM.02430-06View
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Abstract

Biological Sciences Agricultural and Veterinary Sciences Medical and Health Sciences
A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

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