Optimising transient assays for studying the regulation of PPO gene expression in pineapple blackheart

Yuchan Zhou; M Jobin-Decor; J Dahler; R Wills; Steven J R Underhill; M W Graham

doi:10.17660/ActaHortic.2002.575.70

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Optimising transient assays for studying the regulation of PPO gene expression in pineapple blackheart

Journal article

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Optimising transient assays for studying the regulation of PPO gene expression in pineapple blackheart

Yuchan Zhou, M Jobin-Decor, J Dahler, R Wills, Steven J R Underhill and M W Graham

Acta Horticulturae, Vol.575, pp.595-601

International Symposium on Tropical and Subtropical Fruits (Cairns, Australia, 25-Nov-2000–01-Dec-2000)

2002

DOI: https://doi.org/10.17660/ActaHortic.2002.575.70

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Abstract

To study factors influencing the development of pineapple blackheart we are undertaking functional analyses of a polyphenol oxidase promoter whose expression is induced in blackheart affected tissues. Since transgenic pineapple fruit take three years to develop, such an analysis must be undertaken using transient assays. In this report we describe experiments aimed at optimising a transient assay system using biolistic DNA delivery in pineapple fruit. Two factors are shown to be critical, namely the use of unmethylated plasmid DNAs prepared using dam E. coli host strains, and the use of an internal standard to control for the inherent variability of transient expression assays.

Details

Title: Optimising transient assays for studying the regulation of PPO gene expression in pineapple blackheart
Authors: Yuchan Zhou (Author)
M Jobin-Decor (Author)
J Dahler (Author)
R Wills (Author)
Steven J R Underhill (Author)
M W Graham (Author)
Contributors: R Drew (Editor)
Publication details: Acta Horticulturae, Vol.575, pp.595-601
Conference details: International Symposium on Tropical and Subtropical Fruits (Cairns, Australia, 25-Nov-2000–01-Dec-2000)
Publisher: International Society for Horticultural Science
Date published: 2002
DOI: 10.17660/ActaHortic.2002.575.70
ISSN: 0567-7572
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; Sustainability Research Cluster
Language: English
Record Identifier: 99449213902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Agronomy; Biotechnology & Applied Microbiology; Horticulture

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