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Published VersionCC BY V4.0, Open Access
Journal article
Ontogeny of the Cytochrome P450 Superfamily in the Ornate Spiny Lobster (Panulirus ornatus)
International Journal of Molecular Sciences, Vol.25(2), pp.1-18
2024
Abstract
Cytochrome P450s (CYP450s) are a versatile superfamily of enzymes known to undergo rapid evolution. They have important roles across growth and development pathways in crustaceans, although it is difficult to characterise orthologs between species due to their sequence diversity. Conserved CYP450s enzymes in crustaceans are those associated with ecdysteroidogenesis: synthesising and breaking down the active moult hormone, 20-hydroxyecdysone. The complex life cycle of the ornate spiny lobster, Panulirus ornatus, relies on moulting in order to grow and develop. Many of these diverse life stages have been analysed to establish a comprehensive transcriptomic database for this species. The transcripts putatively encoding for CYP450s were mapped using transcriptomic analysis and identified across growth and development stages. With the aid of phylogeny, 28 transcripts of 42 putative P. ornatus CYP450s were annotated, including the well conserved Halloween genes, which are involved in ecdysteroidogenesis. Expression patterns across the life stages determined that only a subset of the CYP450s can be detected in each life stage or tissue. Four Shed transcripts show overlapping expression between metamorphosis and adult tissues, suggesting pleotropic functions of the multiple Shed orthologs within P. ornatus.
Details
- Title
- Ontogeny of the Cytochrome P450 Superfamily in the Ornate Spiny Lobster (Panulirus ornatus)
- Authors
- Courtney L. Lewis (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationQuinn P. Fitzgibbon (Author) - University of TasmaniaGregory G. Smith (Author) - University of TasmaniaAbigail Elizur (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- International Journal of Molecular Sciences, Vol.25(2), pp.1-18
- Publisher
- MDPI AG
- Date published
- 2024
- DOI
- 10.3390/ijms25021070
- ISSN
- 1422-0067; 1422-0067
- Copyright note
- © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- The data presented in this study are openly available in the NCBI SRA database (PRJNA761502, PRJNA877712, PRJNA903480) and can also be found on CrustyBase.org, accessed on 9 January 2024.
- Organisation Unit
- School of Education and Tertiary Access; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99992698702621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
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