Journal article
Ontogenetic variation in digestive enzyme activities within embryos and newly-hatched larvae of the tropical spiny lobster, Panulirus ornatus
Aquaculture, Vol.548, pp.1-8
2022
Abstract
While there is considerable interest in aquaculture of spiny lobsters (Palinuridae), limited knowledge of nutritional requirements during early life stages remains a major impediment to successful hatchery culture. To better determine endogenous nutrient utilisation during the early life stages of the tropical spiny lobster, Panulirus ornatus, we examined ontogenetic variation in digestive enzyme activities within developing embryos and newly-hatched phyllosoma in the absence of an exogenous food source. Specifically, α-amylase, non-specific esterase, and trypsin-like protease activities were quantified to indicate the relative importance of macronutrients (i.e., carbohydrate, lipid, and protein) and gauge the digestive potential of newly-hatched phyllosoma during transition from endogenous to exogenous nutrient sources. Ontogenetic variation in digestive enzyme activities indicated that lipid, protein, and carbohydrate were sequentially utilised by embryos. Early increase in non-specific esterase activity suggests that lipid is the most critical macronutrient for early embryonic development, with protein and carbohydrate becoming increasingly utilised shortly before and after hatching. The observed peak in trypsin-like protease activity during the first day post-hatch presumably allows phyllosoma to make best use of dietary items that are high in protein at first-feeding. In the absence of an exogenous food source after hatching, changes in digestive enzyme activities suggest that a metabolic shift from protein to lipid occurred, coinciding with the timing of the ‘point-of-no-return’ for Stage I phyllosoma of P. ornatus. Our results highlight the importance of providing an exogenous protein-rich food source to phyllosoma soon after hatch and provide a foundation for further study into broodstock nutrition and egg quality of this species.
•Digestive enzyme activities were examined in Panulirus ornatus embryos and larvae.
•Significant ontogenetic variation in enzyme activities occurred.
•Esterase, protease, and amylase activities increased sequentially in embryos.
•Total activities of esterase and protease were highest during the first day post-hatch.
•In absence of exogenous nutrition, larvae shift from protein to lipid utilisation.
Details
- Title
- Ontogenetic variation in digestive enzyme activities within embryos and newly-hatched larvae of the tropical spiny lobster, Panulirus ornatus
- Authors
- Jerome Genodepa (Author) - James Cook UniversityChaoshu Zeng (Corresponding Author) - James Cook UniversityThane A Militz (Author) - University of the Sunshine Coast, Queensland, Australian Centre for Pacific Islands ResearchPaul C Southgate (Author) - University of the Sunshine Coast, Queensland, Australian Centre for Pacific Islands Research
- Publication details
- Aquaculture, Vol.548, pp.1-8
- Publisher
- Elsevier BV
- DOI
- 10.1016/j.aquaculture.2021.737595
- ISSN
- 1873-5622
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Australian Centre for Pacific Islands Research
- Language
- English
- Record Identifier
- 99581208902621
- Output Type
- Journal article
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