Journal article
Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom
Toxicon, Vol.44(6), pp.635-640
2004
Abstract
Venom-yield and composition of differently sized individuals of the medically most important Brazilian spider Phoneutria nigriventer (Keyserling, 1891) was analysed. During growth the venom-mass increases according to a fourth order function of the prosoma size, which mainly reflects an increase of the venom gland volume. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed increasing percentages of proteins ≤17 kDa from 4.1% in the smallest analysed spiders (2-3 months-old) to 79.1% in adult female venom. Additionally, high-pressure liquid-chromatography showed an increase of a single ('main') peak from 4.6 to 64.9%, while the overall number of other major-peaks decreased. Venom from young instars completely lacked lethality in mice up to a dose of 3.28 mg/kg i.v. as compared to a LD50 of 0.63 mg/kg for adult female or 1.57 mg/kg for adult male venom that we reported previously. In conclusion, ontogenetic changes in venom protein-composition of growing P. nigriventer are suggested to produce increasing lethality in vertebrates.
Details
- Title
- Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom
- Authors
- Volker Herzig (Author) - University of Tubingen, GermanyR J Ward (Author) - FFCLRP-USP, BrazilW F Dos Santos (Author) - FFCLRP-USP, Brazil
- Publication details
- Toxicon, Vol.44(6), pp.635-640
- Publisher
- Elsevier Ltd.
- Date published
- 2004
- DOI
- 10.1016/j.toxicon.2004.07.020
- ISSN
- 0041-0101
- Copyright note
- Copyright © 2004 Elsevier Science Ltd. All rights reserved. Reproduced here with kind permission of the publisher.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451100002621
- Output Type
- Journal article
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