Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm

Lara T Meital; Mark Windsor; Maria-Christina Perissiou; Karl Schulze; Rebecca Magee; Anna V Kuballa; Jonathan Golledge; Tom G Bailey; Christopher D Askew; Fraser D Russell

doi:10.1038/s41598-019-49362-z

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Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm

Journal article

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Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm

Lara T Meital, Mark Windsor, Maria-Christina Perissiou, Karl Schulze, Rebecca Magee, Anna V Kuballa, Jonathan Golledge, Tom G Bailey, Christopher D Askew and Fraser D Russell

Scientific Reports, Vol.9, pp.1-11

2019

DOI: https://doi.org/10.1038/s41598-019-49362-z

PMCID: PMC6736886

PMID: 31506475

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Abstract

Abdominal aortic aneurysm (AAA) is associated with inflammation and oxidative stress, the latter of which contributes to activation of macrophages, a prominent cell type in AAA. Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to limit oxidative stress in animal models of AAA. The aim of this study was to evaluate the effect of the n-3 PUFA docosahexaenoic acid (DHA) on antioxidant defence in macrophages from patients with AAA. Cells were obtained from men with small AAA (diameter 3.0-4.5 cm, 75±6 yr, n= 19) and age- matched male controls (72±5 yr, n= 41) and incubated with DHA for 1 h before exposure to 0.1 µg/mL lipopolysaccharide (LPS) for 24 h. DHA supplementation decreased the concentration of tumour necrosis factor-α (TNF-α; control, 42.1±13.6 to 5.1±2.1 pg/ml, p < 0.01; AAA, 25.2±9.8 to 1.9±0.9 pg/ml, p < 0.01) and interleukin-6 (IL-6; control, 44.9±7.7 to 5.9±2.0 pg/ml, p < 0.001; AAA, 24.3±5.2 to 0.5±0.3 pg/ml, p < 0.001) in macrophage supernatants. DHA increased glutathione peroxidase activity (control, 3.2±0.3 to 4.1±0.2 nmol/min/ml/μg protein, p = 0.004; AAA, 2.3±0.5 to 3.4±0.5 nmol/min/ml/μg protein, p = 0.008) and heme oxygenase-1 mRNA expression (control, 1.5-fold increase, p < 0.001). The improvements in macrophage oxidative stress status serve as a stimulus for further investigation of DHA in patients with AAA.

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Title: Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm
Authors: Lara T Meital (Author) - University of the Sunshine Coast
Mark Windsor (Author) - University of the Sunshine Coast
Maria-Christina Perissiou (Author) - University of the Sunshine Coast
Karl Schulze (Author) - Sunshine Vascular
Rebecca Magee (Author) - Sunshine Coast University Hospital
Anna V Kuballa (Author) - University of the Sunshine Coast
Jonathan Golledge (Author) - James Cook University
Tom G Bailey (Author) - University of the Sunshine Coast
Christopher D Askew (Author) - University of the Sunshine Coast
Fraser D Russell (Author) - University of the Sunshine Coast
Publication details: Scientific Reports, Vol.9, pp.1-11
Publisher: Nature Publishing Group
Date published: 2019
DOI: 10.1038/s41598-019-49362-z
ISSN: 2045-2322
PMID: 31506475; PMC6736886
Copyright note: Copyright © 2019 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Grants: Developing improved management for peripheral artery diseases, 1117061, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Organisation Unit: School of Health - Biomedicine; UniSC Clinical Trials Centre; School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99451378502621
Output Type: Journal article

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