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Odorant receptor orthologues in conifer -feeding beetles display conserved responses to ecologically relevant odours741.74 kB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Odorant receptor orthologues in conifer-feeding beetles display conserved responses to ecologically relevant odors
Molecular Ecology, Vol.31(13), pp.3693-3707
2022
PMID: 35532927
Abstract
Insects are able to detect a plethora of olfactory cues using a divergent family of odorant receptors (ORs). Despite the divergent nature of this family, related species frequently express several evolutionarily conserved OR orthologues. In the largest order of insects, Coleoptera, it remains unknown whether OR orthologues have conserved or divergent functions in different species. Using HEK293 cells, we addressed this question through functional characterization of two groups of OR orthologues in three species of the Curculionidae (weevil) family, the conifer-feeding bark beetles Ips typographus L. (‘Ityp’) and Dendroctonus ponderosae Hopkins (‘Dpon’) (Scolytinae), and the pine weevil Hylobius abietis L. (‘Habi’; Molytinae). The ORs of H. abietis were annotated from antennal transcriptomes. Results show highly conserved response specificities, with one group of orthologues (HabiOR3/DponOR8/ItypOR6) responding exclusively to 2-phenylethanol (2-PE), and the other group (HabiOR4/DponOR9/ItypOR5) responding to angiosperm green leaf volatiles (GLVs). Both groups of orthologues belong to the coleopteran OR subfamily 2B, and share a common ancestor with OR5 in the cerambycid Megacyllene caryae, also tuned to 2-PE, suggesting a shared evolutionary history of 2-PE receptors across two beetle superfamilies. The detected compounds are ecologically relevant for conifer-feeding curculionids, and are probably linked to fitness, with GLVs being used to avoid angiosperm non-host plants, and 2-PE being important for intraspecific communication and/or playing a putative role in beetle-microbe symbioses. To our knowledge, this study is the first to reveal evolutionary conservation of OR functions across several beetle species and hence sheds new light on the functional evolution of insect ORs.
Details
- Title
- Odorant receptor orthologues in conifer-feeding beetles display conserved responses to ecologically relevant odors
- Authors
- Rebecca E Roberts (Author) - Lund UniversityTwinkle Biswas (Author) - Lund UniversityJothi Kumar Yuvaraj (Author) - Lund UniversityEwald Grosse-Wilde (Author) - Czech University of Life Sciences PragueDaniel Powell (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringBill S Hansson (Author) - Max Planck Institute for Chemical EcologyChrister Löfstedt (Author) - Lund UniversityMartin N Andersson (Corresponding Author) - Lund University
- Publication details
- Molecular Ecology, Vol.31(13), pp.3693-3707
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2022
- DOI
- 10.1111/mec.16494
- ISSN
- 1365-294X; 0962-1083
- PMID
- 35532927
- Copyright note
- © 2022 The Authors. Molecular Ecology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made
- Grant note
- Vetenskapsrådet (2017‐03804) Svenska Forskningsrådet Formas (2018‐01444; 2018‐01630; 217‐2014‐689) Carl Tryggers Stiftelse för Vetenskaplig Forskning (CTS 17:25) Max‐Planck‐Gesellschaft Kungliga Fysiografiska Sällskapet i Lund Crafoordska Stiftelsen Stiftelsen Oscar och Lili Lamms Minne
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99637978702621
- Output Type
- Journal article
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