Journal article
Novel aromatic polymers for immobilizing B-D-glucosidase and their possible application to cellulolysis
Applied Biochemistry and Biotechnology, Vol.9(4), p.413
1984
Abstract
We have synthesized, by enzymic and chemical means, a variety of novel polyaromatic-enzyme complexes that are extremely stable and show promise in the conversion of cellulose to glucose. Thus we have prepared a number of homo- and heteropolymeric supports (involving l-tyrosine, pyrogallol, resorcinol, phloroglucinol, orcinol, catechol, protocatechuic acid, and various hydroxybenzoic acids) and discovered that, for example, a resorcinol-?-d-glucosidase copolymer has high stability combined with low Km (10.5 m M vs commercial soluble (3-d-glucosidase 9.3 mM) and high Vmax values (104 ?mol ?NP mg-1H-1 vs 85 ?mol ?NP mg-1H-1). These properties are enhanced when the copolymer is complexed with bentonite clay. The kinetic constants of the resorcinol-?-d-glucosidase copolymer-bentonite complex were Km = 9.6 m M and Vmax = 73.5 ?mol ?NP mg-1H-1. Stability has been assessed against proteolysis, organic solvents, elevated temperatures, storage, and incorporation into fresh soil. A cellulase preparation from Trichoderma viride has also been copolymerized with a variety of phenolic macromolecules and displays varying degrees of stability and activity against carboxymethyl cellulose. The resorcinol ?-d-glucosidase-copolymer was immobilized on a PM10 ultrafiltration membrane (Km = 16.8 mM; Vmax = 42.4 (?mol ?NP mg-1H-1) and showed enhanced thermostability, a broader pH range for maximal activity, and could be reused without loss of activity. An ultrafiltration cell, containing the membrane-immobilized resorcinol-?-d-glucosida se copolymer, can be operated as a continuous reactor with substrate flow rates from 0.1 to 0.7 mL min-1 without decrease in product formation. © 1984 The Humana Press Inc.
Details
- Title
- Novel aromatic polymers for immobilizing B-D-glucosidase and their possible application to cellulolysis
- Authors
- J M Sarkar (Author) - University of Kent, United KingdomRichard G Burns (Author) - University of Kent, United Kingdom
- Publication details
- Applied Biochemistry and Biotechnology, Vol.9(4), p.413
- Publisher
- Humana Press, Inc
- Date published
- 1984
- DOI
- 10.1007/BF02799002
- ISSN
- 0273-2289
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
- Language
- English
- Record Identifier
- 99449279602621
- Output Type
- Journal article
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- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology