Novel Microsatellite Markers for Conservation of Australian Native Samadera bidwillii

Vatsal M Naik; Nanjappa Ashwath; Robert W Lamont; Alison Shapcott

doi:10.4236/oje.2018.82006

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Novel Microsatellite Markers for Conservation of Australian Native Samadera bidwillii

Journal article

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Novel Microsatellite Markers for Conservation of Australian Native Samadera bidwillii

Vatsal M Naik, Nanjappa Ashwath, Robert W Lamont and Alison Shapcott

Open Journal of Ecology, Vol.8(2), pp.75-85

2018

DOI: https://doi.org/10.4236/oje.2018.82006

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Abstract

Plant Biology

Genetics

454 sequencing

conservation

microsatellites

samadera

native shrub

Microsatellite markers were developed for Samadera bidwillii, a nationally listed vulnerable shrub or small tree, to enable investigation of its genetic structure and diversity within and among populations from its known distribution throughout coastal areas mainly in fragmented and disturbed lands from Mackay to Gympie, Queensland, Australia. The loci were tested for cross-amplification in related Samadera species. Ten polymorphic microsatellite markers were isolated and characterised from an enriched library of S. bidwillii, which exhibited di- and trinucleotide repeats. The mean number of alleles per locus ranged from 1.3 to 2.5 and mean expected and observed heterozygosities ranged from 0.06 to 0.33 and from 0.03 to 0.26, respectively in five populations. All loci successfully amplified in six other closely associated Samadera species also reported from Australia. Developed loci can be used in genetic diversity, population structure and gene flow studies with an emphasis on the conservation of S. bidwillii and related species.

Details

Title: Novel Microsatellite Markers for Conservation of Australian Native Samadera bidwillii
Authors: Vatsal M Naik (Author) - Central Queensland University
Nanjappa Ashwath (Author) - Central Queensland University
Robert W Lamont (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Alison Shapcott (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Open Journal of Ecology, Vol.8(2), pp.75-85
Publisher: Scientific Research Publishing
Date published: 2018
DOI: 10.4236/oje.2018.82006
ISSN: 2162-1985
Copyright note: Copyright © 2018 by authors and Scientific Research Publishing Inc.This work is licensed under the Creative Commons Attribution International License (CC BY 4.0).http://creativecommons.org/licenses/by/4.0/
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; School of Law and Society; Centre for Bioinnovation
Language: English
Record Identifier: 99450672402621
Output Type: Journal article

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