NextGen molecular barcoding of larval grouper diet in an extensive green-water pond system

Lachlan Dennis; Kelli Anderson; Matthew J Wylie; Vu In; Josephine Nocillado; Abigail Elizur

doi:10.1016/j.aquaculture.2020.735971

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NextGen molecular barcoding of larval grouper diet in an extensive green-water pond system

Journal article

Peer reviewed

NextGen molecular barcoding of larval grouper diet in an extensive green-water pond system

Lachlan Dennis, Kelli Anderson, Matthew J Wylie, Vu In, Josephine Nocillado and Abigail Elizur

Aquaculture, Vol.531, pp.1-11

2021

DOI: https://doi.org/10.1016/j.aquaculture.2020.735971

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Abstract

Molecular barcoding and next generation amplicon sequencing were used to investigate the diet of the larvae of two species: tiger (Epinephelus fuscoguttatus) and hybrid (E. lanceolatus × E. fuscoguttatus) grouper. Larvae were reared in open green-water ponds. These ponds had natural populations of wild zooplankton, and were also seeded with additional zooplankton. Zooplankton from water samples and larvae were collected daily for the first 12 days post hatch (dph) for hybrid grouper and the first 6 dph for tiger grouper. Next generation (NextGen) sequencing with degenerate cytochrome oxidase 1 oligonucleotide primers was performed on all samples. Resulting sequences suggest that both grouper species predated heavily on calanoid copepods – especially at first feeding and while still drawing energy from endogenous supplies. In addition, tiger grouper consumed mussel trochophores as a first feed. Grouper larvae diversified their diets at 6 dph and hybrid grouper were found to be consuming an unidentified insect as their primary food source from this point. Diets further diversified at 10 dph and the first substantial representation of ‘traditional’ aquaculture live feed was seen at 12 dph with the appearance of Artemia. Rotifers (Brachionus spp.) were consumed at a low level by tiger grouper larvae and at a negligible level by hybrid grouper despite daily addition to the pond systems. This study confirms that within the first 12 dph calanoid copepods are the key prey zooplankton for grouper larvae, and demonstrates that NextGen sequencing is a powerful tool for diet assessment in aquaculture.

Details

Title: NextGen molecular barcoding of larval grouper diet in an extensive green-water pond system
Authors: Lachlan Dennis (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Kelli Anderson (Author) - University of Tasmania
Matthew J Wylie (Author) - New Zealand Institute for Plant and Food Research Limited
Vu In (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Josephine Nocillado (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Abigail Elizur (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: Aquaculture, Vol.531, pp.1-11
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2020.735971
ISSN: 1873-5622
Organisation Unit: School of Science, Technology and Engineering; University of the Sunshine Coast, Queensland; Centre for Bioinnovation; School of Science and Engineering - Legacy
Language: English
Record Identifier: 99480408702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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