Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study

Faith O. Alele; John R. Otto; Bunmi S. Malau-Aduli; Aduli E. O. Malau-Aduli

doi:10.3390/biom12101465

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Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study

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Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study

Faith O. Alele, John R. Otto, Bunmi S. Malau-Aduli and Aduli E. O. Malau-Aduli

Biomolecules, Vol.12(10), pp.1-13

2022

DOI: https://doi.org/10.3390/biom12101465

PMID: 36291674

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Abstract

heat tolerance

heat shock proteins

HSPA1B

HSP90AA2

exertional heat stroke

exertional heat illness

military

genetics

polymorphism

Heat tolerance and exertional heat stroke (EHS) are rare health conditions that have been described and characterised but have never been genetically solved. Knowledge of the role of single nucleotide polymorphisms (SNPs) in heat shock proteins (HSPs) genes and their associations with heat tolerance and EHS is limited. This pilot study aimed to identify SNP in HSPA1B, HSP90AA2 and DNAJA1 genes and their associations with heat tolerance and EHS history in a quasi-experimental design. Participants comprised Australian Defence Force members (ADF) who had a history of EHS and the general population. Genomic DNA samples were extracted from the venous blood samples of 48 participants, sequenced and analysed for SNP. Forty-four per cent (44%) of the participants were heat intolerant, and 29% had a history of EHS. Among participants with a history of EHS, there was an association between heat tolerance and HSPA1B SNP at the g.31829044 locus. However, there were no associations between HSPA1B and HSP90AA2 SNP and heat tolerance. All participants had the same distribution for the DNAJA1 SNP. In conclusion, the findings indicate an association between the HSPA1B genetic variant at the g.31829044 locus and heat tolerance among ADF participants with a history of EHS. Further research with a larger number of military participants will shed more light on the associations between HSP genes and heat tolerance.

Details

Title: Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study
Authors: Faith O. Alele (Author) - James Cook University
John R. Otto (Author) - James Cook University
Bunmi S. Malau-Aduli (Author) - James Cook University
Aduli E. O. Malau-Aduli (Corresponding Author) - James Cook University
Publication details: Biomolecules, Vol.12(10), pp.1-13
Publisher: MDPI AG
Date published: 2022
DOI: 10.3390/biom12101465
ISSN: 2218-273X
PMID: 36291674
Copyright note: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health - Public Health
Language: English
Record Identifier: 99967596602621
Output Type: Journal article

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