Neuropeptides encoded by the genomes of the Akoya pearl oyster Pinctata fucata and Pacific oyster Crassostrea gigas: A bioinformatic and peptidomic survey

Michael J Stewart; Pascal Favrel; Bronwyn Rotgans; Tianfang Wang; Min Zhao; Manzar Sohail; Wayne A O'Connor; Abigail Elizur; Joel Henry; Scott F Cummins

doi:10.1186/1471-2164-15-840

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Neuropeptides encoded by the genomes of the Akoya pearl oyster Pinctata fucata and Pacific oyster Crassostrea gigas: A bioinformatic and peptidomic survey

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Neuropeptides encoded by the genomes of the Akoya pearl oyster Pinctata fucata and Pacific oyster Crassostrea gigas: A bioinformatic and peptidomic survey

Michael J Stewart, Pascal Favrel, Bronwyn Rotgans, Tianfang Wang, Min Zhao, Manzar Sohail, Wayne A O'Connor, Abigail Elizur, Joel Henry and Scott F Cummins

BMC Genomics, Vol.15, 840

2014

DOI: https://doi.org/10.1186/1471-2164-15-840

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Abstract

pinctada fucata

crassostrea gigas

molluscs

circular dichroism

egg-laying hormone

feed circuit activating peptide

gonadotropin-releasing hormone

high-performance liquid chromatography

mass spectrometry

neuropeptides

Background: Oysters impart significant socio-ecological benefits from primary production of food supply, to estuarine ecosystems via reduction of water column nutrients, plankton and seston biomass. Little though is known at the molecular level of what genes are responsible for how oysters reproduce, filter nutrients, survive stressful physiological events and form reef communities. Neuropeptides represent a diverse class of chemical messengers, instrumental in orchestrating these complex physiological events in other species. Results By a combination of in silico data mining and peptide analysis of ganglia, 74 putative neuropeptide genes were identified from genome and transcriptome databases of the Akoya pearl oyster, Pinctata fucata and the Pacific oyster, Crassostrea gigas, encoding precursors for over 300 predicted bioactive peptide products, including three newly identified neuropeptide precursors PFGx8amide, RxIamide and Wx3Yamide. Our findings also include a gene for the gonadotropin-releasing hormone (GnRH) and two egg-laying hormones (ELH) which were identified from both oysters. Multiple sequence alignments and phylogenetic analysis supports similar global organization of these mature peptides. Computer-based peptide modeling of the molecular tertiary structures of ELH highlights the structural homologies within ELH family, which may facilitate ELH activity leading to the release of gametes. Conclusion Our analysis demonstrates that oysters possess conserved molluscan neuropeptide domains and overall precursor organization whilst highlighting many previously unrecognized bivalve idiosyncrasies. This genomic analysis provides a solid foundation from which further studies aimed at the functional characterization of these molluscan neuropeptides can be conducted to further stimulate advances in understanding the ecology and cultivation of oysters.

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Title: Neuropeptides encoded by the genomes of the Akoya pearl oyster Pinctata fucata and Pacific oyster Crassostrea gigas: A bioinformatic and peptidomic survey
Authors: Michael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Pascal Favrel (Author) - Université de Caen Basse-Normandie, France
Bronwyn Rotgans (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Tianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Min Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Manzar Sohail (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wayne A O'Connor (Author) - New South Wales Department of Primary Industries
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Joel Henry (Author) - Université de Caen Basse-Normandie, France
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: BMC Genomics, Vol.15, 840
Publisher: BioMed Central Ltd.
Date published: 2014
DOI: 10.1186/1471-2164-15-840
ISSN: 1471-2164
Copyright note: Copyright © 2014 Stewart, etal; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This published version is reproduced in accordance with this policy.
Organisation Unit: Faculty of Science, Health, Education and Engineering; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99448888902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity