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Mutations in the gene encoding GlyT2 (SLC6A5) define a presynaptic component of human startle disease
Journal article   Open access   Peer reviewed

Mutations in the gene encoding GlyT2 (SLC6A5) define a presynaptic component of human startle disease

M I Rees, K Harvey, B R Pearce, S K Chung, I C Duguid, P Thomas, S Beatty, G E Graham, L Armstrong, R Shiang, …
Nature Genetics, Vol.38(7), pp.801-806
2006
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https://doi.org/10.1038/ng1814View
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Abstract

Hyperekplexia is a human neurological disorder characterized by an excessive startle response and is typically caused by missense and nonsense mutations in the gene encoding the inhibitory glycine receptor (GlyR) α1 subunit (GLRA1). Genetic heterogeneity has been confirmed in rare sporadic cases, with mutations affecting other postsynaptic glycinergic proteins including the GlyR β subunit (GLRB), gephyrin (GPHN) and RhoGEF collybistin (ARHGEF9). However, many individuals diagnosed with sporadic hyperekplexia do not carry mutations in these genes. Here we show that missense, nonsense and frameshift mutations in SLC6A5 (ref. 8), encoding the presynaptic glycine transporter 2 (GlyT2), also cause hyperekplexia. Individuals with mutations in SLC6A5 present with hypertonia, an exaggerated startle response to tactile or acoustic stimuli, and life-threatening neonatal apnea episodes. SLC6A5 mutations result in defective subcellular GlyT2 localization, decreased glycine uptake or both, with selected mutations affecting predicted glycine and Na+ binding sites. © 2006 Nature Publishing Group.

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