Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

Francesca Genova; Simona Nonnis; Elisa Maffioli; Gabriella Tedeschi; Maria Giuseppina Strillacci; Michela Carisetti; Giuseppe Sironi; Francesca Anna Cupaioli; Noemi Di Nanni; Alessandra Mezzelani; Ettore Mosca; Christopher R Helps; Peter A J Leegwater; Laetitia Dorso; 99 Lives Consortium; Maria Longeri; Robert Harvey

doi:10.1038/s41598-021-87168-0

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Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

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Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

Francesca Genova, Simona Nonnis, Elisa Maffioli, Gabriella Tedeschi, Maria Giuseppina Strillacci, Michela Carisetti, Giuseppe Sironi, Francesca Anna Cupaioli, Noemi Di Nanni, Alessandra Mezzelani, …

Scientific Reports, Vol.11, pp.1-14

2021

DOI: https://doi.org/10.1038/s41598-021-87168-0

PMID: 33863921

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Abstract

Genomics

data integration

kidney diseases

miRNAs

proteomics

The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of two affected Abyssinians and 195 controls of other breeds (part of the 99 Lives initiative) were screened to prioritize potential disease-associated variants. Proteome and miRNAome from formalin-fixed paraffin-embedded kidney specimens of fully necropsied Abyssinian cats, three affected and three non-amyloidosis-affected were characterized. While the trigger of the disorder remains unclear, overall, (i) 35,960 genomic variants were detected; (ii) 215 and 56 proteins were identified as exclusive or overexpressed in the affected and control kidneys, respectively; (iii) 60 miRNAs were differentially expressed, 20 of which are newly described. With omics data integration, the general conclusions are: (i) the familial amyloid renal form in Abyssinians is not a simple monogenic trait; (ii) amyloid deposition is not triggered by mutated amyloidogenic proteins but is a mix of proteins codified by wild-type genes; (iii) the form is biochemically classifiable as AA amyloidosis.

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Title: Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits
Authors: Francesca Genova (Author) - University of Milan
Simona Nonnis (Author) - University of Milan
Elisa Maffioli (Author) - University of Milan
Gabriella Tedeschi (Author) - University of Milan
Maria Giuseppina Strillacci (Author) - University of Milan
Michela Carisetti (Author) - University of Milan
Giuseppe Sironi (Author) - University of Milan
Francesca Anna Cupaioli (Author) - National Research Council
Noemi Di Nanni (Author) - National Research Council
Alessandra Mezzelani (Author) - National Research Council
Ettore Mosca (Author) - National Research Council
Christopher R Helps (Author) - University of Bristol
Peter A J Leegwater (Author) - Utrecht University
Laetitia Dorso (Author) - Ecole Nationale Vétérinaire de Toulouse
99 Lives Consortium (Research Group)
Maria Longeri (Author) - University of Milan
Robert Harvey (Consortium Member) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Publication details: Scientific Reports, Vol.11, pp.1-14
Publisher: Nature Publishing Group
Date published: 2021
DOI: 10.1038/s41598-021-87168-0
ISSN: 2045-2322
PMID: 33863921
Copyright note: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99522204302621
Output Type: Journal article

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