Journal article
Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus
BMC Genomics, Vol.12, 147
2011
Abstract
Background: Crustacean moulting is a complex process involving many regulatory pathways. A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for Portunus pelagicus. The printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and Y-organ from all moult cycle stages. Results: A total of 556 clones were sequenced from the cDNA libraries used to construct the arrays. These cDNAs represented 175 singlets and 62 contigs, resulting in 237 unique putative genes. The gene sequences were classified into the following biological functions: cuticular proteins associated with arthropod exoskeletons, farnesoic acid O-methyltransferase (FaMeT), proteins belonging to the hemocyanin gene family, lectins, proteins relevant to lipid metabolism, mitochondrial proteins, muscle related proteins, phenoloxidase activators and ribosomal proteins. Moult cycle-related differential expression patterns were observed for many transcripts. Of particular interest were those relating to the formation and hardening of the exoskeleton, and genes associated with cell respiration and energy metabolism. Conclusions:The expression data presented here provide a chronological depiction of the molecular events associated with the biological changes that occur during the crustacean moult cycle. Tracing the temporal expression patterns of a large variety of transcripts involved in the moult cycle of P. pelagicus can provide a greater understanding of gene function, interaction, and regulation of both known and new genes with respect to the moulting process.
Details
- Title
- Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus
- Authors
- Anna V Kuballa (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationT A Holton (Author) - University of QueenslandB Paterson (Author) - Queensland Department of Employment, Economic Development and InnovationAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- BMC Genomics, Vol.12, 147
- Publisher
- BioMed Central Ltd.
- Date published
- 2011
- DOI
- 10.1186/1471-2164-12-147
- ISSN
- 1471-2164
- Copyright note
- Copyright © 2011 Kuballa, et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This published version is reproduced in accordance with this policy.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449452102621
- Output Type
- Journal article
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