Morphological characterization of virus-like particles in coral reef sponges

Cecília Pascelli; Patrick W Laffy; Marija Kupresanin; Timothy Ravasi; Nicole S Webster

doi:10.7717/peerj.5625

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Morphological characterization of virus-like particles in coral reef sponges

Journal article

Open access

Peer reviewed

Morphological characterization of virus-like particles in coral reef sponges

Cecília Pascelli, Patrick W Laffy, Marija Kupresanin, Timothy Ravasi and Nicole S Webster

PeerJ, Vol.6(10), pp.1-21

2018

DOI: https://doi.org/10.7717/peerj.5625

PMCID: PMC6195793

PMID: 30356950

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Abstract

Virus

United Kingdom

Marine sponges

Red Sea

VLP

TEM

Transmission Electron Microscopy

Great Barrier Reef

Marine sponges host complex microbial consortia that vary in their abundance, diversity and stability amongst host species. While our understanding of sponge-microbe interactions has dramatically increased over the past decade, little is known about how sponges and their microbial symbionts interact with viruses, the most abundant entities in the ocean. In this study, we employed three transmission electron microscopy (TEM) preparation methods to provide the first comprehensive morphological assessment of sponge-associated viruses. The combined approaches revealed 50 different morphologies of viral-like particles (VLPs) represented across the different sponge species. VLPs were visualized within sponge cells, within the sponge extracellular mesohyl matrix, on the sponge ectoderm and within sponge-associated microbes. Non-enveloped, non-tailed icosahedral VLPs were the most commonly observed morphotypes, although tailed bacteriophage, brick-shaped, geminate and filamentous VLPs were also detected. Visualization of sponge-associated viruses using TEM has confirmed that sponges harbor not only diverse communities of microorganisms but also diverse communities of viruses.

Details

Title: Morphological characterization of virus-like particles in coral reef sponges
Authors: Cecília Pascelli (Author) - James Cook University
Patrick W Laffy (Author) - Australian Institute of Marine Science
Marija Kupresanin (Author) - King Abdullah University of Science and Technology
Timothy Ravasi (Author) - King Abdullah University of Science and Technology
Nicole S Webster (Author) - James Cook University
Publication details: PeerJ, Vol.6(10), pp.1-21
Publisher: PeerJ, Ltd.
Date published: 2018
DOI: 10.7717/peerj.5625
ISSN: 2167-8359
PMID: 30356950; PMC6195793
Copyright note: Copyright © 2018 Pascelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99569200602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology