Molecular structure of glycogen in diabetic liver

Bin Deng; Mitchell A Sullivan; Jialun Li; Xinle Tan; Chengjun Zhu; Benjamin L Schulz; Robert G. Gilbert

doi:10.1007/s10719-015-9578-6

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Molecular structure of glycogen in diabetic liver

Journal article

Peer reviewed

Molecular structure of glycogen in diabetic liver

Bin Deng, Mitchell A Sullivan, Jialun Li, Xinle Tan, Chengjun Zhu, Benjamin L Schulz and Robert G. Gilbert

Glycoconjugate Journal, Vol.32(3-4), pp.113-118

2015

DOI: https://doi.org/10.1007/s10719-015-9578-6

PMID: 25796617

Abstract

glycogen

db/db mice

size-exclusion chromatography

type 2 diabetes

Liver glycogen (involved in maintaining blood-sugar levels) is a hyperbranched glucose polymer containing beta particles (diameter 20 nm), which can form composite alpha particles (diameter 50-300 nm), and includes a small but significant amount of bound protein. Size distributions of glycogen from livers of healthy and diabetic mice were examined using size-exclusion chromatography with two separate eluents: aqueous eluent and dimethylsulfoxide (DMSO) eluent. Morphologies were examined with transmission electron microscopy. Diabetic glycogen (DG) exhibited many alpha particles in the mild water-based solvent, but in DMSO, which breaks H bonds, these degraded to beta particles; alpha particles however were always present in healthy glycogen (HG). This DG fragility shows the binding of beta into alpha particles is different in HG and DG. The diabetic alpha particle fragility may be involved with the uncontrolled blood-sugar release symptomatic of diabetes: small beta particles degrade more easily to glucose than alpha particles. This has implications for diabetes management.

Details

Title: Molecular structure of glycogen in diabetic liver
Authors: Bin Deng - Huazhong University of Science and Technology
Mitchell A Sullivan - Huazhong University of Science and Technology
Jialun Li - Huazhong University of Science and Technology
Xinle Tan - Huazhong University of Science and Technology
Chengjun Zhu - University of Queensland
Benjamin L Schulz - University of Queensland
Robert G. Gilbert (Corresponding Author) - Huazhong University of Science and Technology
Publication details: Glycoconjugate Journal, Vol.32(3-4), pp.113-118
Publisher: Springer New York LLC
Date published: 2015
DOI: 10.1007/s10719-015-9578-6
ISSN: 1573-4986
PMID: 25796617
Grants: Structural understanding of the degradation of glucose polymers, DP130102461, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991035097802621
Output Type: Journal article