Journal article
Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea
PLoS One, Vol.6(3), e18343
2011
Abstract
Background: The island of New Guinea is located midway between the world's two major melioidosis endemic regions of Australia and Southeast Asia. Previous studies in Papua New Guinea have demonstrated autochthonous melioidosis in Balimo, Western province. In contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. Methodology/Principal Findings: We employed molecular typing techniques to determine the phylogenetic relationships of these isolates to each other and to others worldwide to aid in understanding the origins of the Papua New Guinean isolates. Multi-locus sequence typing of the 39 isolates resolved three unique sequence types. Phylogenetic reconstruction and Structure analysis determined that all isolates were genetically closer to those from Australia than those from Southeast Asia. Gene cluster analysis however, identified a Yersinia-like fimbrial gene cluster predominantly found among Burkholderia pseudomallei derived from Southeast Asia. Higher resolution VNTR typing and phylogenetic reconstruction of the Balimo isolates resolved 24 genotypes with long branch lengths. These findings are congruent with long term persistence in the region and a high level of environmental stability. Conclusions/Significance: Given that anthropogenic influence has been hypothesized as a mechanism for the dispersal of B. pseudomallei, these findings correlate with limited movement of the indigenous people in the region. The palaeogeographical and anthropogenic history of Australasia and the results from this study indicate that New Guinea is an important region for the further study of B. pseudomallei origins and dissemination. © 2011 Baker et al.
Details
- Title
- Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea
- Authors
- A Baker (Author) - James Cook UniversityT Pearson (Author) - Northern Arizona University, United StatesErin P Price (Author) - Northern Arizona University, United StatesJ Dale (Author) - Northern Arizona University, United StatesP Keim (Author) - Northern Arizona University, United StatesH Hornstra (Author) - Northern Arizona University, United StatesA Greenhill (Author) - James Cook UniversityG Padilla (Author) - James Cook UniversityJ Warner (Author) - James Cook University
- Publication details
- PLoS One, Vol.6(3), e18343
- Publisher
- Public Library of Science
- Date published
- 2011
- DOI
- 10.1371/journal.pone.0018343
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2011 Baker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451062202621
- Output Type
- Journal article
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- Microbiology
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