Molecular markers for discriminating Streptococcus pyogenes and S. dysgalactiae subspecies equisimilis

David J McMillan; T Vu; P V Bramhachari; S Y Kaul; A Bouvet; M S Shaila; M G Karmarkar; K S Sriprakash

doi:10.1007/s10096-010-0899-x

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Molecular markers for discriminating Streptococcus pyogenes and S. dysgalactiae subspecies equisimilis

Journal article

Peer reviewed

Molecular markers for discriminating Streptococcus pyogenes and S. dysgalactiae subspecies equisimilis

David J McMillan, T Vu, P V Bramhachari, S Y Kaul, A Bouvet, M S Shaila, M G Karmarkar and K S Sriprakash

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Vol.29(5), pp.585-589

2010

DOI: https://doi.org/10.1007/s10096-010-0899-x

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Abstract

Given the increasing aetiological importance of Streptococcus dysgalactiae subspecies equisimilis in diseases which are primarily attributed to S. pyogenes, molecular markers are essential to distinguish these species and delineate their epidemiology more precisely. Many clinical microbiology laboratories rely on agglutination reactivity and biochemical tests to distinguish them. These methods have limitations which are particularly exacerbated when isolates with mixed properties are encountered. In order to provide additional distinguishing parameters that could be used to unequivocally discriminate these two common pathogens, we assess here three molecular targets: the speB gene, intergenic region upstream of the scpG gene (IRSG) and virPCR. Of these, the former two respectively gave positive and negative results for S. pyogenes, and negative and positive results for S. dysgalactiae subsp. equisimilis. Thus, a concerted use of these nucleic acid-based methods is particularly helpful in epidemiological surveillance to accurately assess the relative contribution of these species to streptococcal infections and diseases.

Details

Title: Molecular markers for discriminating Streptococcus pyogenes and S. dysgalactiae subspecies equisimilis
Authors: David J McMillan (Author) - Queensland Institute of Medical Research
T Vu (Author) - Queensland Institute of Medical Research
P V Bramhachari (Author) - Indian Institute of Science, India
S Y Kaul (Author) - Queensland Institute of Medical Research
A Bouvet (Author) - Université Paris Descartes, France
M S Shaila (Author) - Indian Institute of Science, India
M G Karmarkar (Author) - KEM Hospital, India
K S Sriprakash (Author) - Queensland Institute of Medical Research
Publication details: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Vol.29(5), pp.585-589
Publisher: Springer
Date published: 2010
DOI: 10.1007/s10096-010-0899-x
ISSN: 0934-9723
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450506802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Microbiology

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