Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards

T Ezaz; A E Quinn; S D Sarre; Denis O'Meally; A Georges; J A Marshall Graves

doi:10.1007/s10577-008-9019-5

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Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards

Journal article

Peer reviewed

Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards

T Ezaz, A E Quinn, S D Sarre, Denis O'Meally, A Georges and J A Marshall Graves

Chromosome Research, Vol.17(1), pp.91-98

2009

DOI: https://doi.org/10.1007/s10577-008-9019-5

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Abstract

GSD

TSD

reptile

sex microchromosomes

evolution

FISH

C-banding

Distribution of sex-determining mechanisms across Australian agamids shows no clear phylogenetic segregation, suggesting multiple transitions between temperature-dependent (TSD) and genotypic sex determination (GSD). These taxa thus present an excellent opportunity for studying the evolution of sex chromosomes, and evolutionary transitions between TSD and GSD. Here we report the hybridization of a 3 kb genomic sequence (PvZW3) that marks the Z and W microchromosomes of the Australian central bearded dragon (Pogona vitticeps) to chromosomes of 12 species of Australian agamids from eight genera using fluorescence in-situ hybridization (FISH). The probe hybridized to a single microchromosome pair in 11 of these species, but to the tip of the long arm of chromosome pair 2 in the twelfth (Physignathus lesueurii), indicating a micro-macro chromosome rearrangement. Three TSD species shared the marked microchromosome, implying that it is a conserved autosome in related species that determine sex by temperature. C-banding identified the marked microchromosome as the heterochromatic W chromosome in two of the three GSD species. However, in Ctenophorus fordi, the probe hybridized to a different microchromosome from that shown by C-banding to be the heterochromatic W, suggesting an independent origin for the ZW chromosome pair in that species. Given the haphazard distribution of GSD and TSD in this group and the existence of at least two sets of sex microchromosomes in GSD species, we conclude that sex-determining mechanisms in this family have evolved independently, multiple times in a short evolutionary period. © 2009 Springer Science+Business Media B.V.

Details

Title: Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards
Authors: T Ezaz (Author) - Australian National University
A E Quinn (Author) - University of Canberra
S D Sarre (Author) - University of Canberra
Denis O'Meally (Author) - Australian National University
A Georges (Author) - University of Canberra
J A Marshall Graves (Author) - Australian National University
Publication details: Chromosome Research, Vol.17(1), pp.91-98
Publisher: Springer Netherlands
Date published: 2009
DOI: 10.1007/s10577-008-9019-5
ISSN: 0967-3849
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450449902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Genetics & Heredity

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