Journal article
Molecular genotyping of Acinetobacter spp. isolated in Arizona, USA, using multilocus PCR and mass spectrometry
Journal of Medical Microbiology, Vol.62, pp.1295-1300
2013
Abstract
Acinetobacter spp. are a diverse group of Gram-negative bacteria frequently implicated in nosocomial infections. Genotypic methods have been instrumental in studying Acinetobacter, but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. Here, we further evaluated multilocus PCR electrospray ionization/ mass spectrometry (PCR/ESI-MS), a method that is simple and robust, and provides both species characterization and strain-level resolution of Acinetobacter spp. on a single platform. We examined 125 Acinetobacter isolates from 21 hospitals, laboratories and medical centres spanning four counties in Arizona, USA, using PCR/ESI-MS. We compared PCR/ESI-MS with an in-house amplified fragment length polymorphism (AFLP) genotyping scheme. PCR/ESI-MS demonstrated that Acinetobacter spp. from Arizonan hospitals had similar species and strain distributions to other US civilian hospitals. Furthermore, we showed that the PCR/ESI-MS and AFLP genotypes were highly congruent, with the former having the advantages of robust interlaboratory reproducibility, rapid turnaround time and simple experimental set-up and data analysis. PCR/ESI-MS is an effective and high-throughput platform for strain typing of Acinetobacter baumannii and for identification of other Acinetobacter spp., including the emerging nosocomial pathogens Acinetobacter pittii and Acinetobacter nosocomialis. © 2013 SGM.
Details
- Title
- Molecular genotyping of Acinetobacter spp. isolated in Arizona, USA, using multilocus PCR and mass spectrometry
- Authors
- Derek S Sarovich (Author) - Northern Arizona University, United StatesR E Colman (Author) - Northern Arizona University, United StatesErin P Price (Author) - Northern Arizona University, United StatesC Massire (Author) - IBIS Biosciences, United StatesA T Von Schulze (Author) - Northern Arizona University, United StatesV Waddell (Author) - Arizona Department of Health Services, United StatesS M Anderson (Author) - Arizona Department of Health Services, United StatesD J Ecker (Author) - IBIS Biosciences, United StatesA P Liguori (Author) - Northern Arizona University, United StatesD M Engelthaler (Author) - Translational Genomics Research Institute, United StatesR Sampath (Author) - IBIS Biosciences, United StatesP Keim (Author) - Northern Arizona University, United StatesM W Eshoo (Author) - IBIS Biosciences, United StatesD M Wagner (Author) - Northern Arizona University, United States
- Publication details
- Journal of Medical Microbiology, Vol.62, pp.1295-1300
- Publisher
- Microbiology Society
- Date published
- 2013
- DOI
- 10.1099/jmm.0.052381-0
- ISSN
- 0022-2615
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450705902621
- Output Type
- Journal article
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