Molecular evidence for novel chlamydial infections in the koala (Phascolarctos cinereus)

L N Devereaux; Adam Polkinghorne; A Meijer; Peter Timms

doi:10.1078/072320203322346092

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Molecular evidence for novel chlamydial infections in the koala (Phascolarctos cinereus)

Journal article

Peer reviewed

Molecular evidence for novel chlamydial infections in the koala (Phascolarctos cinereus)

L N Devereaux, Adam Polkinghorne, A Meijer and Peter Timms

Systematic and Applied Microbiology, Vol.26(2), pp.245-253

2003

DOI: https://doi.org/10.1078/072320203322346092

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Abstract

chlamydophila pecorum

chlamydophila pneumoniae

uncultured koala chlamydiales

chlamydia - like

Chlamydia-related disease has a detrimental effect on Australia's free-range koala (Phascolarctos cinereus) populations. The chlamydial species responsible for ocular, urogenital and respiratory disease in the koala have previously been identified as Chlamydophila pecorum and Chlamydophila pneumoniae. Epizootiology studies have therefore used species specific PCR assays to detect chlamydial infections. In the current study, we used a broad range PCR amplification and cloning strategy to identify all strains of Chlamydiales in the koala. Sequencing of 16S rRNA gene PCR products, cloned from Chlamydiales - order positive swab samples identified nine novel koala Chlamydiales genotypes, including multiple novel chlamydial genotypes present in a single sample. The novel koala genotypes are clustered together with other Chlamydia-like bacteria within a second lineage separate from the known Chlamydiaceae species. Two new primer sets UKC-A and UKC-B were designed to detect five of the nine novel Chlamydiales and were applied to swab samples collected from two wild koala populations. Using these new UKC PCR assays, UKC-A type Chlamydiales sequences were more prevalent (72%; 18/25) compared to UKC-B (24%; 6/25). UKC sequences were most commonly found as dual infections with C.pecorum. This report provides the first description of additional members of the order Chlamydiales infecting the koala.

Details

Title: Molecular evidence for novel chlamydial infections in the koala (Phascolarctos cinereus)
Authors: L N Devereaux (Author) - Queensland University of Technology
Adam Polkinghorne (Author) - Queensland University of Technology
A Meijer (Author) - National Institute of Public Health and the Environment, Netherlands
Peter Timms (Author) - Queensland University of Technology
Publication details: Systematic and Applied Microbiology, Vol.26(2), pp.245-253
Publisher: Elsevier GmbH - Urban und Fischer
Date published: 2003
DOI: 10.1078/072320203322346092
ISSN: 0723-2020
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449851002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology

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