Journal article
Molecular epidemiology of third-generation cephalosporin-resistant Enterobacteriaceae in Southeast Queensland, Australia
Antimicrobial Agents and Chemotherapy, Vol.65(6)
2021
PMCID: PMC8315908
PMID: 33781999
Abstract
Third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae represent a major threat to human health. Here, we captured 288 3GC-R Enterobacteriaceae clinical isolates from 264 patients presenting at a regional Australian hospital over a 14-month period. Alongside routine mass spectrometry speciation and antibiotic sensitivity testing, isolates were examined using rapid (∼40 min) real-time PCR assays targeting the most common extended spectrum β-lactamases (ESBLs;
and
groups, plus
,
, and an internal 16S ribosomal DNA control). AmpC CMY β-lactamase (
) prevalence was also examined.
(80.2%) and
(17.0%) were dominant, with
,
and
infrequently identified. Ceftriaxone and cefoxitin resistance were identified in 97.0% and 24.5% of
and
isolates, respectively. Consistent with global findings in Enterobacteriaceae, most (98.3%) isolates harbored at least one β-lactamase gene, with 144 (50%) encoding
group, 92 (31.9%)
group, 48 (16.7%)
, 133 (46.2%)
, and 34 (11.8%)
A subset of isolates (
=98) were subjected to whole-genome sequencing (WGS) to identify the presence of cryptic resistance determinants, and to verify genotyping accuracy. WGS of β-lactamase negative or carbapenem-resistant isolates identified uncommon ESBLs and carbapenemases, including
and
, and confirmed all PCR-positive genotypes. We demonstrate that our PCR assays enable the rapid and cost-effective identification of ESBLs in the hospital setting, which has important infection control and therapeutic implications.
Details
- Title
- Molecular epidemiology of third-generation cephalosporin-resistant Enterobacteriaceae in Southeast Queensland, Australia
- Authors
- Adam G Stewart (Author) - The University of QueenslandErin P Price (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyKim Schabacker (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyMehmet Birikmen (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyP N A Harris (Author) - The University of QueenslandK Choong (Author) - Sunshine Coast University Hospital (Australia)S Subedi (Author) - Sunshine Coast University Hospital (Australia)Derek S Sarovich (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Antimicrobial Agents and Chemotherapy, Vol.65(6)
- Publisher
- American Society for Microbiology
- Date published
- 2021
- DOI
- 10.1128/AAC.00130-21
- ISSN
- 1098-6596
- PMID
- 33781999; PMC8315908
- Organisation Unit
- University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99522507102621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Microbiology
- Pharmacology & Pharmacy
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