Journal article
Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction
DNA Sequence, Vol.2(2), pp.121-123
1991
Abstract
The complementary DNA (cDNA) encoding the preprotein growth hormone (pre-GH) from two Australian marine fish species, namely Acanthopagrus butcheri and Lates calcarifer, have been isolated, cloned and sequenced. The sequences were amplified from reverse transcribed total RNA of whole brains using Polymerase Chain Reaction (PCR) and oligonucleotide primers corresponding to the 5' and 3' regions of Pagrus major. Use of PCR offers a rapid method of isolating fish GH cDNA sequences for commercial and taxonomic applications. Sequence comparison indicates a high degree of conservation for GH cDNAs within the family Sparidae.
Details
- Title
- Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction
- Authors
- Wayne R Knibb (Author) - University of AdelaideA Robins (Author) - University of AdelaideL Crocker (Author) - University of AdelaideJ Rizzon (Author) - University of AdelaideA Heyward (Author) - University of AdelaideJ Wells (Author) - University of Adelaide
- Publication details
- DNA Sequence, Vol.2(2), pp.121-123
- Publisher
- Informa Healthcare
- Date published
- 1991
- DOI
- 10.3109/10425179109039680
- ISSN
- 1940-1736
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449771102621
- Output Type
- Journal article
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