Molecular characterization of a vitellogenesis-inhibiting hormone (VIH) in the mud crab (Scylla olivacea) and temporal changes in abundances of VIH mRNA transcripts during ovarian maturation and following neurotransmitter administration

Napamanee Kornthong; Supawadee Duangprom; Saowaros Suwansa-ard; Jirawat Saetan; Teva Phanaksri; Sineenart Songkoomkrong; Supawadee Kheowakae; Jutaporn Pollawat; Prasert Sobhon

doi:10.1016/j.anireprosci.2019.106122

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Molecular characterization of a vitellogenesis-inhibiting hormone (VIH) in the mud crab (Scylla olivacea) and temporal changes in abundances of VIH mRNA transcripts during ovarian maturation and following neurotransmitter administration

Journal article

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Molecular characterization of a vitellogenesis-inhibiting hormone (VIH) in the mud crab (Scylla olivacea) and temporal changes in abundances of VIH mRNA transcripts during ovarian maturation and following neurotransmitter administration

Napamanee Kornthong, Supawadee Duangprom, Saowaros Suwansa-ard, Jirawat Saetan, Teva Phanaksri, Sineenart Songkoomkrong, Supawadee Kheowakae, Jutaporn Pollawat and Prasert Sobhon

Animal Reproduction Science, Vol.208, pp.1-12

2019

DOI: https://doi.org/10.1016/j.anireprosci.2019.106122

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Abstract

vitellogenesis-inhibiting hormone

ovarian maturation

mud crab

neurotransmitters neurohormone

The vitellogenesis-inhibiting hormone (VIH), also known as gonad-inhibiting hormone, is a neuropeptide hormone in crustaceans that belongs to the crustacean hyperglycemic hormone (CHH)-family peptide. There is regulation vitellogenesis by VIH during gonad maturation in crustaceans. A full-length Scylla olivacea VIH (Scyol-VIH) was identified through reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. The open reading frame consists of 378 nucleotides, which encodes a 126-amino acid precursor protein, including a 22-residue signal peptide and a 103-amino acid mature peptide in which 6 highly conserved cysteine residues are present. There was expression of the Scyol-VIH gene in immature female Scylla olivacea in the eyestalk, brain and ventral nerve cord. The Scyol-VIH gene expression was localized to the eyestalk X-organ, brain neuronal clusters 6 and 11, and in multiple neuronal clusters of the ventral nerve cord. The relative abundance of Scyol-VIH mRNA transcript in the eyestalk was relatively greater in immature stage females, then decreased as ovarian maturation progressed. Furthermore, eyestalk Scyol-VIH increased after dopamine (5 μg/g BW) injection. The present research provides fundamental information about Scyol-VIH and its potential effect in controlling reproduction.

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Title: Molecular characterization of a vitellogenesis-inhibiting hormone (VIH) in the mud crab (Scylla olivacea) and temporal changes in abundances of VIH mRNA transcripts during ovarian maturation and following neurotransmitter administration
Authors: Napamanee Kornthong (Author) - Thammasat University, Thailand
Supawadee Duangprom (Author) - Thammasat University, Thailand
Saowaros Suwansa-ard (Author) - University of the Sunshine Coast
Jirawat Saetan (Author) - Prince of Songkla University, Thailand
Teva Phanaksri (Author) - Thammasat University, Thailand
Sineenart Songkoomkrong (Author) - Thammasat University, Thailand
Supawadee Kheowakae (Author) - Thammasat University, Thailand
Jutaporn Pollawat (Author) - Thammasat University, Thailand
Prasert Sobhon (Author) - Mahidol University, Thailand
Publication details: Animal Reproduction Science, Vol.208, pp.1-12
Publisher: Elsevier BV
Date published: 2019
DOI: 10.1016/j.anireprosci.2019.106122
ISSN: 0378-4320
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450861302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences

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