Journal article
Molecular characterization of DNA double strand breaks with tip-enhanced Raman scattering
Angewandte Chemie - International Edition, Vol.53(1), pp.169-172
2014
Abstract
DNA double strand breaks (DSBs) are deadly lesions that can lead to genetic defects and cell apoptosis. Techniques that directly detect DNA DSBs include scanning electron microscopy, atomic force microscopy (AFM), and fluorescence based approaches. While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip-enhanced Raman scattering (TERS) can provide molecular information from DNA at the nanoscale and in combination with AFM provides a new way to visualize and characterize the molecular structure of DSBs. DSBs result from cleavage at the 3'- and 5'-bonds of deoxyribose upon exposure to UVC radiation based on the observation of P-O-H and methyl/methylene deformation modes enhanced in the TERS spectra. It is hypothesized that strand fragments are hydrogen-terminated at the lesion, indicating the action of free radicals during photon exposure. Individual DNA double strand breaks (DSBs) were first detected and located by atomic force microscopy, and the molecular structure of this damage was characterized with tip-enhanced Raman scattering (see picture) using a top-down configuration and a reflective substrate. The first experimental evidence is reported confirming that individual DSBs result from cleavage at the 3′- and 5′-bonds of deoxyribose upon exposure to ultraviolet C radiation.
Details
- Title
- Molecular characterization of DNA double strand breaks with tip-enhanced Raman scattering
- Authors
- E Lipiec (Author) - Henryk Niewodniczanski Institute of Nuclear Physics PAN, PolandRyo Sekine (Author) - University of South AustraliaJ Bielecki (Author) - Henryk Niewodniczanski Institute of Nuclear Physics PAN, PolandW M Kwiatek (Author) - Henryk Niewodniczanski Institute of Nuclear Physics PAN, PolandB R Wood (Author) - Monash University
- Publication details
- Angewandte Chemie - International Edition, Vol.53(1), pp.169-172
- Publisher
- Wiley - V C H Verlag GmbH & Co. KGaA
- Date published
- 2014
- DOI
- 10.1002/anie.201307271
- ISSN
- 1433-7851
- Copyright note
- This is the peer reviewed version of the following article: Lipiec, E., Sekine, R., Bielecki, J., Kwiatek, W.M. and Wood, B.R. (2014), Molecular Characterization of DNA Double Strand Breaks with Tip-Enhanced Raman Scattering. Angew. Chem. Int. Ed., 53: 169-172. https://doi.org/10.1002/anie.201307271, which has been published in final form at https://doi.org/10.1002/anie.201307271. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99450845002621
- Output Type
- Journal article
Metrics
117 File views/ downloads
55 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Multidisciplinary
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites