Molecular characterization of "Candidatus Similichlamydia latridicola" gen. nov., sp. nov. (Chlamydiales: "Candidatus Parilichlamydiaceae"), a novel Chlamydia-like epitheliocystis agent in the striped trumpeter, Latris lineata (Forster)

M C Stride; Adam Polkinghorne; T L Miller; B F Nowak

doi:10.1128/AEM.00746-13

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Molecular characterization of "Candidatus Similichlamydia latridicola" gen. nov., sp. nov. (Chlamydiales: "Candidatus Parilichlamydiaceae"), a novel Chlamydia-like epitheliocystis agent in the striped trumpeter, Latris lineata (Forster)

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Molecular characterization of "Candidatus Similichlamydia latridicola" gen. nov., sp. nov. (Chlamydiales: "Candidatus Parilichlamydiaceae"), a novel Chlamydia-like epitheliocystis agent in the striped trumpeter, Latris lineata (Forster)

M C Stride, Adam Polkinghorne, T L Miller and B F Nowak

Applied and Environmental Microbiology, Vol.79(16), pp.4914-4920

2013

DOI: https://doi.org/10.1128/AEM.00746-13

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Abstract

16S rRNA sequences

histological analysis

in-situ hybridization

molecular analysis

molecular characterization

phylogenetic analysis

sequence divergences

Tasmania

Australia

biotechnology

microbiology

bacteria

bacterial RNA

RNA 16S

aquaculture

Histological analysis of gill samples taken from individuals of latris lineata reared in aquaculture in tasmania, australia, and those sampled from the wild revealed the presence of epitheliocystis-like basophilic inclusions. subsequent morphological, in situ hybridization, and molecular analyses were performed to confirm the presence of this disease and discovered a chlamydialike organism associated with this condition, and the criteria set by fredericks and relman's postulates were used to establish disease causation. three distinct 16s rrna genotypes were sequenced from 16 fish, and phylogenetic analyses of the nearly fulllength 16s rrna sequences generated for this bacterial agent indicated that they were nearly identical novel members of the order chlamydiales. this new taxon formed a well-supported clade with "candidatus parilichlamydia carangidicola" from the yellowtail kingfish (seriola lalandi). on the basis of sequence divergence over the 16s rrna region relative to all other members of the order chlamydiales, a new genus and species are proposed here for the chlamydia-like bacterium from l. lineata, i.e., "candidatus similichlamydia latridicola" gen. nov., sp. nov. © 2013, American Society for Microbiology.

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Title: Molecular characterization of "Candidatus Similichlamydia latridicola" gen. nov., sp. nov. (Chlamydiales: "Candidatus Parilichlamydiaceae"), a novel Chlamydia-like epitheliocystis agent in the striped trumpeter, Latris lineata (Forster)
Authors: M C Stride (Author) - University of Tasmania
Adam Polkinghorne (Author) - Queensland University of Technology
T L Miller (Author) - James Cook University
B F Nowak (Author) - University of Tasmania
Publication details: Applied and Environmental Microbiology, Vol.79(16), pp.4914-4920
Publisher: American Society for Microbiology
Date published: 2013
DOI: 10.1128/AEM.00746-13
ISSN: 0099-2240
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99448877502621
Output Type: Journal article

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