Molecular characterization of "Candidatus Parilichlamydia carangidicola," a novel Chlamydia-Like epitheliocystis agent in yellowtail kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, "Candidatus Parilichlamydiaceae" fam. nov. (order Chlamydiales)

M C Stride; Adam Polkinghorne; T L Miller; J M Groff; S E LaPatra; B F Nowaka

doi:10.1128/AEM.02899-12

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Molecular characterization of "Candidatus Parilichlamydia carangidicola," a novel Chlamydia-Like epitheliocystis agent in yellowtail kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, "Candidatus Parilichlamydiaceae" fam. nov. (order Chlamydiales)

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Molecular characterization of "Candidatus Parilichlamydia carangidicola," a novel Chlamydia-Like epitheliocystis agent in yellowtail kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, "Candidatus Parilichlamydiaceae" fam. nov. (order Chlamydiales)

M C Stride, Adam Polkinghorne, T L Miller, J M Groff, S E LaPatra and B F Nowaka

Applied and Environmental Microbiology, Vol.79(5), pp.1590-1597

2013

DOI: https://doi.org/10.1128/AEM.02899-12

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Abstract

16S rRNA

16S rRNA sequences

Arctic charrs

Atlantic salmon

cytoplasmic vacuolization

epithelial cells

molecular characterization

morphological features

phylogenetic analysis

rRNA sequences

South Australia

RNA

transmission electron microscopy

Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported "Candidatus Piscichlamydia salmonis" (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydialike bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as "Candidatus Parilichlamydia carangidicola" and that the new family be known as "Candidatus Parilichlamydiaceae". © 2013, American Society for Microbiology.

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Title: Molecular characterization of "Candidatus Parilichlamydia carangidicola," a novel Chlamydia-Like epitheliocystis agent in yellowtail kingfish, Seriola lalandi (Valenciennes), and the proposal of a new family, "Candidatus Parilichlamydiaceae" fam. nov. (order Chlamydiales)
Authors: M C Stride (Author) - University of Tasmania
Adam Polkinghorne (Author) - Queensland University of Technology
T L Miller (Author) - James Cook University
J M Groff (Author) - University of California, United States
S E LaPatra (Author) - Clear Springs Food Inc., United States
B F Nowaka (Author) - University of Tasmania
Publication details: Applied and Environmental Microbiology, Vol.79(5), pp.1590-1597
Publisher: American Society for Microbiology
Date published: 2013
DOI: 10.1128/AEM.02899-12
ISSN: 0099-2240
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99447757702621
Output Type: Journal article

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