Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

S Panasophonkul; S Apisawetakan; Scott F Cummins; P S York; B M Degnan; P J Hanna; P Saitongdee; P Sobhon; P Sretarugsa

doi:10.1007/s00418-009-0555-7

Back

Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Journal article

Peer reviewed

Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

S Panasophonkul, S Apisawetakan, Scott F Cummins, P S York, B M Degnan, P J Hanna, P Saitongdee, P Sobhon and P Sretarugsa

Histochemistry and Cell Biology, Vol.131(5), pp.629-642

2009

DOI: https://doi.org/10.1007/s00418-009-0555-7

Files and links (1)

url

https://doi.org/10.1007/s00418-009-0555-7View

Published Version

Abstract

serotonin receptor

abalone

ganglia

gonads

In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 ha shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 ha is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 ha belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 ha mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 ha specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 ha immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.

Details

Title: Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
Authors: S Panasophonkul (Author)
S Apisawetakan (Author)
Scott F Cummins (Author) - University of Queensland
P S York (Author)
B M Degnan (Author)
P J Hanna (Author)
P Saitongdee (Author)
P Sobhon (Author)
P Sretarugsa (Author)
Publication details: Histochemistry and Cell Biology, Vol.131(5), pp.629-642
Publisher: Springer
Date published: 2009
DOI: 10.1007/s00418-009-0555-7
ISSN: 0948-6143
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449704902621
Output Type: Journal article

Metrics

2 File views/ downloads

485 Record Views

18 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Cell Biology; Microscopy