Molecular characterisation of the Australian and New Zealand livestock Chlamydia pecorum strains confirms novel but clonal ST23 in association with ovine foetal loss

Martina Jelocnik; Rhys T. White; Tom Clune; John O’Connell; Jonathan Foxwell; Sam Hair; Shane Besier; La Tom; Nyree Phillips; Amy Robbins; Daniel Bogema; Paola K. Vaz; Alistair R. Legione; Cheryl Jenkins; Caroline Jacobson

doi:10.1016/j.vetmic.2023.109774

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Molecular characterisation of the Australian and New Zealand livestock Chlamydia pecorum strains confirms novel but clonal ST23 in association with ovine foetal loss

Journal article

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Molecular characterisation of the Australian and New Zealand livestock Chlamydia pecorum strains confirms novel but clonal ST23 in association with ovine foetal loss

Martina Jelocnik, Rhys T. White, Tom Clune, John O’Connell, Jonathan Foxwell, Sam Hair, Shane Besier, La Tom, Nyree Phillips, Amy Robbins, …

Veterinary Microbiology, Vol.283, pp.1-11

2023

DOI: https://doi.org/10.1016/j.vetmic.2023.109774

PMID: 37216721

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Abstract

abortion

Chlamydia pecorum

genomics

molecular epidemiology

sheep

ST23

Chlamydia pecorum is a veterinary pathogen associated with abortions and perinatal mortality in sheep. Recent studies investigating foetal and perinatal lamb mortality in sheep from Australia and New Zealand identified C. pecorum clonal sequence type (ST)23 strains in aborted and stillborn lambs. Presently, there is limited genotypic information on C. pecorum strains associated with reproductive disease, although whole genome sequencing (WGS) of one abortigenic ST23 C. pecorum strain identified unique features, including a deletion in the CDS1 locus of the chlamydial plasmid. We applied WGS on two ST23 strains detected in aborted and stillborn lambs from Australia and used phylogenetic and comparative analyses to compare these to the other available C. pecorum genomes. To re-evaluate the genetic diversity of contemporary strains, we applied C. pecorum genotyping, and chlamydial plasmid sequencing to a range of C. pecorum positive samples and isolates from ewes, aborted foetuses and stillborn lambs, cattle and a goat from diverse geographical regions across Australia and New Zealand.The two new C. pecorum genomes are nearly identical to the genome of the Australian abortigenic strain including the unique deletion in the chlamydial plasmid. Genotyping revealed that these novel C. pecorum ST23 strains are widespread and associated with sheep abortions on Australian and New Zealand farms. In addition, a goat C. pecorum strain (denoted ST 304) from New Zealand was also characterised. This study expands the C. pecorum genome catalogue and describes a comprehensive molecular characterisation of the novel livestock ST23 strains associated with foetal and lamb mortality.

Details

Title: Molecular characterisation of the Australian and New Zealand livestock Chlamydia pecorum strains confirms novel but clonal ST23 in association with ovine foetal loss
Authors: Martina Jelocnik (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Rhys T. White (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Tom Clune (Author) - Murdoch University
John O’Connell (Author) - Ministry for Primary Industries
Jonathan Foxwell (Author) - Ministry for Primary Industries
Sam Hair (Author) - Department of Primary Industries and Regional Development
Shane Besier (Author) - Department of Primary Industries and Regional Development
La Tom (Author) - Murdoch University
Nyree Phillips (Author) - Murdoch University
Amy Robbins (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Daniel Bogema (Author) - New South Wales Department of Primary Industries
Paola K. Vaz (Author) - The University of Melbourne
Alistair R. Legione (Author) - The University of Melbourne
Cheryl Jenkins (Author) - New South Wales Department of Primary Industries
Caroline Jacobson (Author) - Murdoch University
Publication details: Veterinary Microbiology, Vol.283, pp.1-11
Publisher: Elsevier BV
Date published: 2023
DOI: 10.1016/j.vetmic.2023.109774
ISSN: 1873-2542
PMID: 37216721
Grant note: National Collaborative Research Infrastructure Strategy (NCRIS)
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99728798002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology; Veterinary Sciences

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