Journal article
Molecular characterisation of a pH-gated chloride channel from Sarcoptes scabiei
Invertebrate Neuroscience, Vol.7(3), pp.149-156
2007
Abstract
Reports of ivermectin resistance in scabies mites raise concerns regarding the sustainability of mass intervention programs for scabies worldwide and for the treatment of crusted scabies. Ligand gated ion channels (LGICs) are the primary targets of ivermectin in invertebrates. We report the molecular characterisation of SsCl-a novel LGIC from Sarcoptes scabiei var. hominis. While SsCl shows sequence similarity to other LGICs, phylogenetic analysis does not suggest strong homology to conventional glutamate, histamine or GABA gated channels. Instead, it is most similar to Drosophila pH-sensitive and group 1 clades. When expressed in Xenopus oocytes, SsCl forms a homomeric, pH-gated chloride channel that is irreversibly activated by ivermectin. These results provide the first confirmation that this group of LGIC exists in arachnids, and suggest that SsCl may be an in vivo target of ivermectin in S. scabiei.
Details
- Title
- Molecular characterisation of a pH-gated chloride channel from Sarcoptes scabiei
- Authors
- Kate E Mounsey (Author) - Charles Darwin UniversityJ A Dent (Author) - Charles Darwin UniversityD C Holt (Author) - Charles Darwin UniversityJ McCarthy (Author) - Charles Darwin UniversityB J Currie (Author) - Charles Darwin UniversityShelley F Walton (Author) - Charles Darwin University
- Publication details
- Invertebrate Neuroscience, Vol.7(3), pp.149-156
- Publisher
- Springer
- Date published
- 2007
- DOI
- 10.1007/s10158-007-0050-6
- ISSN
- 1354-2516
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449649802621
- Output Type
- Journal article
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