Molecular aspects of eye development and regeneration in the Australian redclaw crayfish, Cherax quadricarinatus

Tomer Ventura; Michael J Stewart; Jennifer Chandler; Bronwyn Rotgans; Abigail Elizur; Alex W Hewitt

doi:10.1016/j.aaf.2018.04.001

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Molecular aspects of eye development and regeneration in the Australian redclaw crayfish, Cherax quadricarinatus

Journal article

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Molecular aspects of eye development and regeneration in the Australian redclaw crayfish, Cherax quadricarinatus

Tomer Ventura, Michael J Stewart, Jennifer Chandler, Bronwyn Rotgans, Abigail Elizur and Alex W Hewitt

Aquaculture and Fisheries, Vol.4(1), pp.27-36

2019

DOI: https://doi.org/10.1016/j.aaf.2018.04.001

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Abstract

ocular development

regeneration

compound eye

crustacean

Cherax quadricarinatus

transcriptome

The compound eye evolved over 500 million years ago and enables mosaic vision in most arthropod species. The molecular regulation of the development of the compound eye has been primarily studied in the fruit fly Drosophila melanogaster. However, due to the nature of holometabolous insects halting growth after their terminal metamorphosis into the adult form, they lack the capacity to regenerate. Crustaceans, unlike holometabolous insects, continue to grow during adulthood, achieved through regular shedding of their exoskeleton, in a cyclic process known as molting. This therefore offers crustaceans as a highly suitable model to study ocular regeneration in the adult arthropod eye. We have assessed the regenerative capacity of the retinal section of the Cherax quadricarinatus (red-claw crayfish) eye, following ablation and successive post-metamorphic molts. This work then provides a transcriptomic description of the outer, pigmented retinal tissue (the ommatidia and lamina ganglionaris) and the basal, non-pigmented neuroendocrine ocular tissue (the X-organ Sinus Gland complex, hemiellipsoid body and optic nerve). Using comparative analysis, we identified all the transcripts in the C. quadricarinatus ocular transcriptome that are known to function in compound eye development in D. melanogaster. Differentially and uniquely transcribed genes of the retina are described, suggesting proposed mechanisms that may regulate ocular regeneration in decapod Crustacea. This research exemplifies the application C. quadricarinatus holds as an optimal model to study the regulation of ocular regeneration. Further in-depth transcriptomic analyses are now required, sampled throughout the regeneration process to better define the regulatory mechanism.

Details

Title: Molecular aspects of eye development and regeneration in the Australian redclaw crayfish, Cherax quadricarinatus
Authors: Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Michael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Jennifer Chandler (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Bronwyn Rotgans (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Alex W Hewitt (Author) - University of Western Australia
Publication details: Aquaculture and Fisheries, Vol.4(1), pp.27-36
Publisher: Elsevier BV
Date published: 2019
DOI: 10.1016/j.aaf.2018.04.001
ISSN: 2468-550X
Copyright note: Copyright © 2018 Published by Elsevier B.V. on behalf of Shanghai Ocean University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: Faculty of Science, Health, Education and Engineering; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451002002621
Output Type: Journal article

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