Molecular analysis of two FMRFamide-encoding transcripts expressed during the development of the tropical abalone Haliotis asinina

Scott F Cummins; A Tollenaere; B M Degnan; R P Croll

doi:10.1002/cne.22621

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Molecular analysis of two FMRFamide-encoding transcripts expressed during the development of the tropical abalone Haliotis asinina

Journal article

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Molecular analysis of two FMRFamide-encoding transcripts expressed during the development of the tropical abalone Haliotis asinina

Scott F Cummins, A Tollenaere, B M Degnan and R P Croll

Journal of Comparative Neurology, Vol.519(10), pp.2043-2059

2011

DOI: https://doi.org/10.1002/cne.22621

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Abstract

neuropeptide

evolution

larval

veliger

trochophore

vestigastropod

gastropod

mollusc

Lophotrochozoa

FMRFamide-related peptides (FaRPs) are involved in numerous neural functions across the animal kingdom and serve as important models for understanding the evolution of neuropeptides. Gastropod molluscs have proven to be particularly useful foci for such studies, but the developmental expression of FaRPs and the evolution of specific transcripts for different peptides are unclear within the molluscs. Here we show that FaRPs are encoded by two transcripts that appear to be splice variants of a single gene in the abalone, Haliotis asinina, which represents the basal vetigastropods. Has-FMRF1 comprises 1438 nucleotides and encodes a precursor protein of 329 amino acids that can potentially produce two copies of FLRFamide, one copy each of TLAGDSFLRFamide, QFYRIamide, SDPDLDDVIRASLLAYSLDDSPNN and SVATAPVEAKAVEAGNKDIE, and 13 copies of FMRFamide. The second 1241 nucleotide transcript, Has-FMRF2, encodes a 206 amino acid precursor protein with single copies of FLRFamide and FMRFamide along with such extended forms as NFGEPFLRFamide, FDSYEDKALRFamide, and NGWLHFamide, in addition to SDPGEDMLKSILLRGAPSNNGLQY and DTUDETTUNDNAHSRQ. Both transcripts are present early in life and expressed in different but overlapping patterns within the developing larval nervous system. Mass spectrometry and immunocytochemistry demonstrate that FaRPs are cleaved from larger precursors and localize to the developing nervous system. Our results confirm previous evidence that FaRPs are expressed early and potentially play many roles during molluscan development and suggest that the last common ancestor to living gastropods used alternative splicing of a FMRFamide gene to generate a diversity of FaRPs in spatially restricted patterns in the nervous system.

Details

Title: Molecular analysis of two FMRFamide-encoding transcripts expressed during the development of the tropical abalone Haliotis asinina
Authors: Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
A Tollenaere (Author) - University of Queensland
B M Degnan (Author) - University of Queensland
R P Croll (Author) - Dalhousie University
Publication details: Journal of Comparative Neurology, Vol.519(10), pp.2043-2059
Publisher: John Wiley & Sons Inc.
Date published: 2011
DOI: 10.1002/cne.22621
ISSN: 0021-9967
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449703402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Neurosciences; Zoology