Molecular Structure of Human-Liver Glycogen

Bin Deng; Mitchell A Sullivan; Cheng Chen; Jialun Li; Prudence O. Powell; Zhenxia Hu; Robert G. Gilbert

doi:10.1371/journal.pone.0150540

Back

Molecular Structure of Human-Liver Glycogen

Journal article

Open access

Peer reviewed

Molecular Structure of Human-Liver Glycogen

Bin Deng, Mitchell A Sullivan, Cheng Chen, Jialun Li, Prudence O. Powell, Zhenxia Hu and Robert G. Gilbert

PLoS One, Vol.11(3), pp.1-10

2016

DOI: https://doi.org/10.1371/journal.pone.0150540

PMID: 26934359

Files and links (1)

pdf

file614.19 kBDownload View

Published VersionCC BY V4.0, Open Access

Abstract

Glycogen is a highly branched glucose polymer which is involved in maintaining blood-sugar homeostasis. Liver glycogen contains large composite α particles made up of linked β particles. Previous studies have shown that the binding which links β particles into α particles is impaired in diabetic mice. The present study reports the first molecular structural characterization of human-liver glycogen from non-diabetic patients, using transmission electron microscopy for morphology and size-exclusion chromatography for the molecular size distribution; the latter is also studied as a function of time during acid hydrolysis in vitro, which is sensitive to certain structural features, particularly glycosidic vs. proteinaceous linkages. The results are compared with those seen in mice and pigs. The molecular structural change during acid hydrolysis is similar in each case, and indicates that the linkage of β into α particles is not glycosidic. This result, and the similar morphology in each case, together imply that human liver glycogen has similar molecular structure to those of mice and pigs. This knowledge will be useful for future diabetes drug targets.

Details

Title: Molecular Structure of Human-Liver Glycogen
Authors: Bin Deng - Huazhong University of Science and Technology
Mitchell A Sullivan - The University of Queensland
Cheng Chen - Wuhan General Hospital of Guangzhou
Jialun Li - Wuhan Union Hospital
Prudence O. Powell - Huazhong University of Science and Technology
Zhenxia Hu - Huazhong University of Science and Technology
Robert G. Gilbert (Corresponding Author) - The University of Queensland
Publication details: PLoS One, Vol.11(3), pp.1-10
Publisher: Public Library of Science
Date published: 2016
DOI: 10.1371/journal.pone.0150540
ISSN: 1932-6203
PMID: 26934359
Copyright note: © 2016 Deng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Grants: Structural understanding of the degradation of glucose polymers, DP130102461, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991035097502621
Output Type: Journal article

Metrics

23 Record Views

34 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites