Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Joseph L Pegler; Jackson M J Oultram; Christopher P L Grof; Andrew Eamens

doi:10.3390/plants10010073

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Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Journal article

Open access

Peer reviewed

Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Joseph L Pegler, Jackson M J Oultram, Christopher P L Grof and Andrew Eamens

Plants, Vol.10(1), pp.1-22

2020

DOI: https://doi.org/10.3390/plants10010073

PMID: 33396498

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Abstract

Arabidopsis thaliana

microRNA399 (miR399)

PHOSPHATE2 (PHO2) gene expression regulation

RT-qPCR

salt stress

Environmental stress

microRNAs

Small RNA biology

In Arabidopsis thaliana (Arabidopsis), the microRNA399 (miR399)/PHOSPHATE2 (PHO2) expression module is central to the response of Arabidopsis to phosphate (PO4) stress. In addition, miR399 has been demonstrated to also alter in abundance in response to salt stress. We therefore used a molecular modification approach to alter miR399 abundance to investigate the requirement of altered miR399 abundance in Arabidopsis in response to salt stress. The generated transformant lines, MIM399 and MIR399 plants, with reduced and elevated miR399 abundance respectively, displayed differences in their phenotypic and physiological response to those of wild-type Arabidopsis (Col-0) plants following exposure to a 7-day period of salt stress. However, at the molecular level, elevated miR399 abundance, and therefore, altered PHO2 target gene expression in salt-stressed Col-0, MIM399 and MIR399 plants, resulted in significant changes to the expression level of the two PO4 transporter genes, PHOSPHATE TRANSPORTER1;4 (PHT1;4) and PHT1;9. Elevated PHT1;4 and PHT1;9 PO4 transporter levels in salt stressed Arabidopsis would enhance PO4 translocation from the root to the shoot tissue which would supply additional levels of this precious cellular resource that could be utilized by the aerial tissues of salt stressed Arabidopsis to either maintain essential biological processes or to mount an adaptive response to salt stress.

Details

Title: Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana
Authors: Joseph L Pegler (Author) - University of Newcastle Australia
Jackson M J Oultram (Author) - University of Newcastle Australia
Christopher P L Grof (Author) - University of Newcastle Australia
Andrew Eamens (Author) - University of Newcastle Australia
Publication details: Plants, Vol.10(1), pp.1-22
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/plants10010073
ISSN: 2223-7747
PMID: 33396498
Copyright note: Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99656593302621
Output Type: Journal article

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