Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress

Joseph L Pegler; Duc Quan Nguyen; Jackson M. J Oultram; Christopher P. L Grof; Andrew L Eamens

doi:10.3390/plants10122570

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Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress

Journal article

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Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress

Joseph L Pegler, Duc Quan Nguyen, Jackson M. J Oultram, Christopher P. L Grof and Andrew L Eamens

Plants , Vol.10(12), pp.1-18

2021

DOI: https://doi.org/10.3390/plants10122570

PMCID: PMC8706208

PMID: 34961041

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Abstract

Arabidopsis thaliana (Arabidopsis)

phosphorous (P)

phosphate (PO4 )

starvation

molecular manipulation

microRNA396 (miR396)

miR399

RT-qPCR gene expression analysis

In plant cells, the molecular and metabolic processes of nucleic acid synthesis, phospholipid production, coenzyme activation and the generation of the vast amount of chemical energy required to drive these processes relies on an adequate supply of the essential macronutrient, phosphorous (P). The requirement of an appropriate level of P in plant cells is evidenced by the intricately linked molecular mechanisms of P sensing, signaling and transport. One such mechanism is the posttranscriptional regulation of the P response pathway by the highly conserved plant microRNA (miRNA), miR399. In addition to miR399, numerous other plant miRNAs are also required to respond to environmental stress, including miR396. Here, we exposed Arabidopsis thaliana (Arabidopsis) transformant lines which harbor molecular modifications to the miR396 and miR399 expression modules to phosphate (PO4) starvation. We show that molecular alteration of either miR396 or miR399 abundance afforded the Arabidopsis transformant lines different degrees of tolerance to PO4 starvation. Furthermore, RT-qPCR assessment of PO4-starved miR396 and miR399 transformants revealed that the tolerance displayed by these plant lines to this form of abiotic stress most likely stemmed from the altered expression of the target genes of these two miRNAs. Therefore, this study forms an early step towards the future development of molecularly modified plant lines which possess a degree of tolerance to growth in a PO4 deficient environment.

Details

Title: Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress
Authors: Joseph L Pegler (Author) - University of Newcastle Australia
Duc Quan Nguyen (Author) - University of Newcastle Australia
Jackson M. J Oultram (Author) - University of Newcastle Australia
Christopher P. L Grof (Author) - University of Newcastle Australia
Andrew L Eamens (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Publication details: Plants , Vol.10(12), pp.1-18
Publisher: MDPI AG
Date published: 2021
DOI: 10.3390/plants10122570
ISSN: 2223-7747
PMID: 34961041; PMC8706208
Copyright note: Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99590208702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Plant Sciences

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