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Molecular manipulation of the mir396 and mir399 expression modules alters the response of arabidopsis thaliana to phosphate stress3.24 MB
Published VersionCC BY-NC V4.0, Open Access
Journal article
Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress
Plants , Vol.10(12), pp.1-18
2021
Abstract
In plant cells, the molecular and metabolic processes of nucleic acid synthesis, phospholipid production, coenzyme activation and the generation of the vast amount of chemical energy required to drive these processes relies on an adequate supply of the essential macronutrient, phosphorous (P). The requirement of an appropriate level of P in plant cells is evidenced by the intricately linked molecular mechanisms of P sensing, signaling and transport. One such mechanism is the posttranscriptional regulation of the P response pathway by the highly conserved plant microRNA (miRNA), miR399. In addition to miR399, numerous other plant miRNAs are also required to respond to environmental stress, including miR396. Here, we exposed Arabidopsis thaliana (Arabidopsis) transformant lines which harbor molecular modifications to the miR396 and miR399 expression modules to phosphate (PO4) starvation. We show that molecular alteration of either miR396 or miR399 abundance afforded the Arabidopsis transformant lines different degrees of tolerance to PO4 starvation. Furthermore, RT-qPCR assessment of PO4-starved miR396 and miR399 transformants revealed that the tolerance displayed by these plant lines to this form of abiotic stress most likely stemmed from the altered expression of the target genes of these two miRNAs. Therefore, this study forms an early step towards the future development of molecularly modified plant lines which possess a degree of tolerance to growth in a PO4 deficient environment.
Details
- Title
- Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress
- Authors
- Joseph L Pegler (Author) - University of Newcastle AustraliaDuc Quan Nguyen (Author) - University of Newcastle AustraliaJackson M. J Oultram (Author) - University of Newcastle AustraliaChristopher P. L Grof (Author) - University of Newcastle AustraliaAndrew L Eamens (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Plants , Vol.10(12), pp.1-18
- Publisher
- MDPI AG
- Date published
- 2021
- DOI
- 10.3390/plants10122570
- ISSN
- 2223-7747
- Copyright note
- Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99590208702621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Plant Sciences
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Source: InCites