Molecular Manipulation of the miR160/AUXIN RESPONSE FACTOR Expression Module Impacts Root Development in Arabidopsis thaliana

Kim Zimmerman; Joseph L. Pegler; Jackson M. J. Oultram; David A. Collings; Ming-Bo Wang; Christopher P. L. Grof; Andrew L. Eamens

doi:10.3390/genes15081042

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Molecular Manipulation of the miR160/AUXIN RESPONSE FACTOR Expression Module Impacts Root Development in Arabidopsis thaliana

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Molecular Manipulation of the miR160/AUXIN RESPONSE FACTOR Expression Module Impacts Root Development in Arabidopsis thaliana

Kim Zimmerman, Joseph L. Pegler, Jackson M. J. Oultram, David A. Collings, Ming-Bo Wang, Christopher P. L. Grof and Andrew L. Eamens

Genes, Vol.15(8), pp.1-23

2024

DOI: https://doi.org/10.3390/genes15081042

PMCID: PMC11353855

PMID: 39202402

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Abstract

Arabidopsis thaliana

Arabidopsis

root development

microRNA (miRNA)

miR160

miR160 expression module

miR160-directed gene expression regulation

Auxin response factor (ARF)

ARF10

ARF16

ARF17

molecular manipulation

In Arabidopsis thaliana (Arabidopsis), microRNA160 (miR160) regulates the expression of AUXIN RESPONSE FACTOR10 (ARF10), ARF16 and ARF17 throughout development, including the development of the root system. We have previously shown that in addition to DOUBLE-STRANDED RNA BINDING1 (DRB1), DRB2 is also involved in controlling the rate of production of specific miRNA cohorts in the tissues where DRB2 is expressed in wild-type Arabidopsis plants. In this study, a miR160 overexpression transgene (MIR160B) and miR160-resistant transgene versions of ARF10 and ARF16 (mARF10 and mARF16) were introduced into wild-type Arabidopsis plants and the drb1 and drb2 single mutants to determine the degree of requirement of DRB2 to regulate the miR160 expression module as part of root development. Via this molecular modification approach, we show that in addition to DRB1, DRB2 is required to regulate the level of miR160 production from its precursor transcripts in Arabidopsis roots. Furthermore, we go on to correlate the altered abundance of miR160 or its ARF10, ARF16 and ARF17 target genes in the generated series of transformant lines with the enhanced development of the root system displayed by these plant lines. More specifically, promotion of primary root elongation likely stemmed from enhancement of miR160-directed ARF17 expression repression, while the promotion of lateral and adventitious root formation was the result of an elevated degree of miR160-directed regulation of ARF17 expression, and to a lesser degree, ARF10 and ARF16 expression. Taken together, the results presented in this study identify the requirement of the functional interplay between DRB1 and DRB2 to tightly control the rate of miR160 production, to in turn ensure the appropriate degree of miR160-directed ARF10, ARF16 and ARF17 gene expression regulation as part of normal root system development in Arabidopsis.

Details

Title: Molecular Manipulation of the miR160/AUXIN RESPONSE FACTOR Expression Module Impacts Root Development in Arabidopsis thaliana
Authors: Kim Zimmerman - University of Newcastle Australia
Joseph L. Pegler - University of Newcastle Australia
Jackson M. J. Oultram - University of Newcastle Australia
David A. Collings - University of Newcastle Australia
Ming-Bo Wang - CSIRO Agriculture and Food (Australia)
Christopher P. L. Grof - University of Newcastle Australia
Andrew L. Eamens (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health - Biomedicine
Publication details: Genes, Vol.15(8), pp.1-23
Publisher: MDPI AG
Date published: 2024
DOI: 10.3390/genes15081042
ISSN: 2073-4425
PMID: 39202402; PMC11353855
Copyright note: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Data Availability: All data reported here are available from the authors upon request.
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991054380102621
Output Type: Journal article

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