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Molecular Manipulation of the MiR396-GRF Expression Module Alters the Salt Stress Response of Arabidopsis thaliana11.64 MB
Published VersionCC BY V4.0, Open Access
Journal article
Molecular Manipulation of the MiR396/GRF Expression Module Alters the Salt Stress Response of Arabidopsis thaliana
Agronomy, Vol.11(9), pp.1-22
2021
Abstract
We previously demonstrated that microRNA396 (miR396) abundance is altered in 15-day-old Arabidopsis thaliana (Arabidopsis) whole seedlings following their exposure to a 7-day salt stress treatment regime. We, therefore, used a molecular modification approach to generate two new Arabidopsis transformant populations with reduced (MIM396 plants) and elevated (MIR396 plants) miR396 abundance. The exposure of 8-day-old wild-type Arabidopsis whole seedlings and a representative plant line of the MIM396 and MIR396 transformant populations to a 7-day salt stress treatment regime revealed unique phenotypic and physiological responses to the imposed stress by unmodified wild-type Arabidopsis plants and the MIM396 and MIR396 transformat lines. A quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) approach was, therefore, applied to demonstrate that the plant line specific responses to salt stress likely stemmed from the unique molecular profile of each of the GROWTH REGULATING FACTOR (GRF) transcription factor gene family members which form posttranscriptional targets of miR396-directed expression regulation. RT-qPCR additionally revealed that, in 15-day-old Arabidopsis whole seedlings, the three previously identified putative target genes of miR396 belonging to the NEUTRAL/ALKALINE NONLYSOSOMAL CERAMIDASE-LIKE (NCER) gene family, including NCER1, NCER2, and NCER3, do not form targets of miR396-directed expression regulation at the posttranscriptional level. Taken together, the phenotypic and molecular analyses presented here demonstrate that alteration of the miR396/GRF expression module is central to the molecular response of Arabidopsis to salt stress.
Details
- Title
- Molecular Manipulation of the MiR396/GRF Expression Module Alters the Salt Stress Response of Arabidopsis thaliana
- Authors
- Joseph L. Pegler (Author) - University of Newcastle AustraliaDuc Quan Nguyen (Author) - University of Newcastle AustraliaJackson M.J. Oultram (Author) - University of Newcastle AustraliaChristopher P.L. Grof (Author) - University of Newcastle AustraliaAndrew L. Eamens (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Agronomy, Vol.11(9), pp.1-22
- Publisher
- MDPI AG
- Date published
- 2021
- DOI
- 10.3390/agronomy11091751
- ISSN
- 2073-4395
- Copyright note
- Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99572908902621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Agronomy
- Plant Sciences
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