Molecular Manipulation of MicroRNA397 Abundance Influences the Development and Salt Stress Response of Arabidopsis thaliana

Duc Quan Nguyen; Christopher W Brown; Joseph L Pegler; Andrew Eamens; Christopher P L Grof

doi:10.3390/ijms21217879

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Molecular Manipulation of MicroRNA397 Abundance Influences the Development and Salt Stress Response of Arabidopsis thaliana

Journal article

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Molecular Manipulation of MicroRNA397 Abundance Influences the Development and Salt Stress Response of Arabidopsis thaliana

Duc Quan Nguyen, Christopher W Brown, Joseph L Pegler, Andrew Eamens and Christopher P L Grof

International Journal of Molecular Sciences, Vol.21(21), pp.1-24

2020

DOI: https://doi.org/10.3390/ijms21217879

PMID: 33114207

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Abstract

Arabidopsis thaliana

Setaria viridis

microRNA397 (miR397)

LACCASE (LAC)

lignin

salt stress

gene expression regulation

RT-qPCR

Arabidopsis thaliana (Arabidopsis) has been used extensively as a heterologous system for molecular manipulation to genetically characterize both dicotyledonous and monocotyledonous plant species. Here, we report on Arabidopsis transformant lines molecularly manipulated to over-accumulate the small regulatory RNA microRNA397 (miR397) from the emerging C4 monocotyledonous grass model species Setaria viridis (S. viridis). The generated transformant lines, termed SvMIR397 plants, displayed a range of developmental phenotypes that ranged from a mild, wild-type-like phenotype, to a severe, full dwarfism phenotype. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR)-based profiling of the SvMIR397 transformant population revealed a strong correlation between the degree of miR397 over-accumulation, repressed LACCASE (LAC) target gene expression, reduced lignin content, and the severity of the developmental phenotype displayed by SvMIR397 transformants. Further, exposure of SvMIR397 transformants to a 7-day regime of salt stress revealed the SvMIR397 transformant lines to be more sensitive to the imposed stress than were wild-type Arabidopsis plants. Taken together, the findings reported here via the use of Arabidopsis as a heterologous system show that the S. viridis miR397 small regulatory RNA is able to repress the expression of three Arabidopsis LAC genes which led to reduced lignin content and increased salt stress sensitivity.

Details

Title: Molecular Manipulation of MicroRNA397 Abundance Influences the Development and Salt Stress Response of Arabidopsis thaliana
Authors: Duc Quan Nguyen (Author) - University of Newcastle Australia
Christopher W Brown (Author) - University of Newcastle Australia
Joseph L Pegler (Author) - University of Newcastle Australia
Andrew Eamens (Author) - University of Newcastle Australia
Christopher P L Grof (Author) - University of Newcastle Australia
Publication details: International Journal of Molecular Sciences, Vol.21(21), pp.1-24
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/ijms21217879
ISSN: 1422-0067; 1661-6596
PMID: 33114207
Copyright note: (c) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99656593202621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary