Journal article
Molecular Insights into Glycogen alpha-Particle Formation
Biomacromolecules, Vol.13(11), pp.3805-3813
2012
PMID: 23004915
Abstract
Glycogen, a hyperbranched complex glucose polymer, is an intracellular glucose store that provides energy for cellular functions, with liver glycogen involved in blood glucose regulation. Liver glycogen comprises complex alpha particles made up of smaller beta particles. The recent discovery that these alpha particles are and fewer. in diabetic, compared: with healthy, mice highlights the need to elucidate the nature of alpha-particle formation, this paper,tests various possibilities for binding within alpha particles. Acid hydrolysis effects, examined using dynamic light scattering and size exclusion.chromatography, showed that the binding is not simple alpha-(1 -> 4) or alpha-(1 -> 6) glycosidic linkages. There was no, significant :change in alpha particle size after the addition of various reagents, Which. disrupt disulfide, protein, and hydrogen bonds and hydrophobic interactions. The results are consistent with proteinaceous binding between beta particles in alpha particles, with the inability Of. protease to break apart particles being attributed to steric. hindrance.
Details
- Title
- Molecular Insights into Glycogen alpha-Particle Formation
- Authors
- Mitchell Sullivan - University of QueenslandMitchell J. O'Connor - University of QueenslandFelipe Umana - University of QueenslandEugeni Roura - University of QueenslandKevin Jack - University of QueenslandDavid Stapleton - University of MelbourneRobert G. Gilbert (Corresponding Author) - University of Queensland
- Publication details
- Biomacromolecules, Vol.13(11), pp.3805-3813
- Publisher
- American Chemical Society
- Date published
- 2012
- DOI
- 10.1021/bm3012727
- ISSN
- 1526-4602
- PMID
- 23004915
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035098702621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Organic
- Polymer Science
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