Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can be Activated by Either γ-Aminobutyric Acid or L-Glutamate

M G Darlison; T Stühmer; Robert J Harvey; F M Dautzenberg; H C Kim; C Zimmermann; M Amar; I Bermudez; J Van Minnen

doi:10.1163/156854293X00557

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Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can be Activated by Either γ-Aminobutyric Acid or L-Glutamate

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Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can be Activated by Either γ-Aminobutyric Acid or L-Glutamate

M G Darlison, T Stühmer, Robert J Harvey, F M Dautzenberg, H C Kim, C Zimmermann, M Amar, I Bermudez and J Van Minnen

Netherlands Journal of Zoology, Vol.44(3), pp.473-485

1993

DOI: https://doi.org/10.1163/156854293X00557

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Abstract

gill and syphon withdrawal reflex

cyclodiene insecticide

ligand-gated ion channel

cDNA cloning

lymnaea stagnalis

xenopus oocyte expression

feeding behaviour

glutamate receptor

in situ hybridisation

GABA receptor

Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be opened by the binding of an appropriate neurotransmitter. Ionotropic receptors for γ-aminobutyric acid (GABA) and L-glutamate have been extensively characterised in the vertebrate nervous system, at the molecular level, through the application of recombinant DNA techniques. These studies have shown that subtypes of GABA receptors (which are inhibitory) and glutamate receptors (which are excitatory) occur, and that these probably all exist in vivo as hetero-oligomers. Although there is ample evidence for the existence of ligand-gated ion channels in invertebrate nervous tissue and muscles, progress in their molecular definition has been comparatively slow. In an effort to determine the nature and specific biological functions of glutamate and GABA receptors in molluscs, we have isolated complementary DNAs (cDNAs), from Lymnaea stagnalis, that encode components of these ion channels. To date, we have obtained full-length cDNAs for three different polypeptides that are similar in sequence to vertebrate GABA type A (GABAA) receptor subunits, and three different polypeptides that resemble vertebrate glutamate-gatcd ion-channel subunits. In addition, we have isolated partial cloncs for five other GABAA receptor-like polypeptides and two further glutamate receptor-like polypeptides. Here we describe the isolation of these clones, and the electrophysiological and pharmacological properties of channels that are formed upon the expression of some of these cDNAs in Xenopus laevis oocytes. © 1993, Brill. All rights reserved.

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Title: Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can be Activated by Either γ-Aminobutyric Acid or L-Glutamate
Authors: M G Darlison (Author) - Universität Hamburg, Germany
T Stühmer (Author) - Universität Hamburg, Germany
Robert J Harvey (Author) - Universität Hamburg, Germany
F M Dautzenberg (Author) - Universität Hamburg, Germany
H C Kim (Author) - Universität Hamburg, Germany
C Zimmermann (Author) - Universität Hamburg, Germany
M Amar (Author) - Universität Hamburg, Germany
I Bermudez (Author) - Oxford Brookes University, United Kingdom
J Van Minnen (Author) - Universiteit, De Boelelaan, Netherlands
Publication details: Netherlands Journal of Zoology, Vol.44(3), pp.473-485
Publisher: Brill
DOI: 10.1163/156854293X00557
ISSN: 0028-2960
Organisation Unit: School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy; School of Health
Language: English
Record Identifier: 99450316802621
Output Type: Journal article

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