Journal article
Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus
The Journal of Physiology, Vol.599(4), pp.1291-1305
2021
PMID: 33135816
Abstract
Mitochondrial dysfunction has been associated with diabetic phenotypes, yet the involvement of placental mitochondria in gestational diabetes mellitus (GDM) remains inconclusive. This is in part complicated by the different mitochondrial subpopulations present in the two major trophoblast cell lineages of the placenta. To better elucidate the role of mitochondria in this pathology, this study examined key aspects of mitochondrial function in placentas from healthy pregnancies and those complicated by GDM in both whole tissue and isolated mitochondria. Mitochondrial content, citrate synthase activity, reactive oxygen species production and gene expression regulating metabolic, hormonal and antioxidant control was examined in placental tissue, before examining functional differences between mitochondrial isolates from cytotrophoblast (Cyto-Mito) and syncytiotrophoblast (Syncytio-Mito). Our study observed evidence of mitochondrial dysfunction across multiple pathways when assessing whole placental tissue from GDM pregnancies compared with healthy controls. Furthermore, by examining isolated mitochondria from the cytotrophoblast and syncytiotrophoblast cell lineages of the placenta we established that although both mitochondrial populations were dysfunctional, they were differentially impacted. These data highlight the need to consider changes in mitochondrial subpopulations at the feto-maternal interface when studying pregnancy pathologies.
Details
- Title
- Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus
- Authors
- Joshua J. Fisher (Corresponding Author) - University of Newcastle AustraliaChelsea L. Vanderpeet (Author) - The University of QueenslandLucy A. Bartho (Author) - Griffith UniversityDaniel R. McKeating (Author) - Griffith UniversityJames S. M. Cuffe (Author) - The University of QueenslandOlivia. J. Holland (Author) - Griffith UniversityAnthony V. Perkins (Author) - Griffith University
- Publication details
- The Journal of Physiology, Vol.599(4), pp.1291-1305
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2021
- DOI
- 10.1113/JP280593
- ISSN
- 1469-7793; 0022-3751
- PMID
- 33135816
- Copyright note
- © 2020 Physiological Society. This is the peer reviewed version of the following article: Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus, The Journal of Physiology, 2020, which has been published in final form at https://doi.org/10.1113/JP280593. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
- Organisation Unit
- School of Health; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99685298702621
- Output Type
- Journal article
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