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Journal of Biophotonics - 2025 - Sleep - Mitochondrial Oxygen Consumption and Immunocytochemistry of Human Dental Pulp Stem961.34 kB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Mitochondrial Oxygen Consumption and Immunocytochemistry of Human Dental Pulp Stem Cell Following 808 nm PBM Therapy: A 3D Cell Culture Study
Journal of Biophotonics, Vol.18(9), pp.1-10
2025
PMID: 40294940
Abstract
This study investigated the impact of 808 nm laser photobiomodulation (PBM) on mitochondrial respiration and osteogenic protein expression (OCN, OPN, ALP, RUNX2, COL-1, BMP-2) in human dental pulp stem cells (hDPSCs) within a 3D hydrogel model. hDPSCs were isolated from third molars and maintained under hypoxic conditions. Cells received PBM at 5 and 15 J/cm2 using an 808 nm diode laser. The study showed that 808 nm PBM can alter mitochondrial respiration, with 5 J/cm2 enhancing osteogenic protein expression (OCN, ALP, OPN, RUNX2) but failing to sustain BMP-2 at 24 h. In contrast, 15 J/cm2 induced stronger upregulation and prolonged BMP-2 expression, suggesting an optimal dose for sustained osteogenic activity. BMP-2 was later downregulated, and COL-1 remained unchanged post-PBM. Importantly, this study indicates the dose-specific PBM modulation of mitochondrial respiration and protein expression, but further research is required to optimize treatment protocols.
Details
- Title
- Mitochondrial Oxygen Consumption and Immunocytochemistry of Human Dental Pulp Stem Cell Following 808 nm PBM Therapy: A 3D Cell Culture Study
- Authors
- Simone L. Sleep - Griffith UniversityEliza Ranjit - Griffith UniversityJennifer Gunter - Queensland University of TechnologyDeanne H. Hryciw - Griffith UniversityPraveen Arany - University at Buffalo, State University of New YorkRoy George (Corresponding Author) - Griffith University
- Publication details
- Journal of Biophotonics, Vol.18(9), pp.1-10
- Publisher
- Wiley-VCH Verlag GmbH & Co. KGaA
- Date published
- 2025
- DOI
- 10.1002/jbio.70051
- ISSN
- 1864-0648; 1864-063X
- PMID
- 40294940
- Copyright note
- © 2025 The Author(s). Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
- Data Availability
- The data that support the findings of this study are available from the corresponding author upon reasonable request.
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991187227702621
- Output Type
- Journal article
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