Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)

J W Ochieng; Dorothy A Steane; P Y Ladiges; P R Baverstock; R J Henry; M Shepherd

doi:10.1590/S1415-47572007000600016

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Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)

Journal article

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Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)

J W Ochieng, Dorothy A Steane, P Y Ladiges, P R Baverstock, R J Henry and M Shepherd

Genetics and Molecular Biology, Vol.30(4), pp.1125-1134

2007

DOI: https://doi.org/10.1590/S1415-47572007000600016

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Abstract

Genetics

microsatellite phylogeny

paraphyletic

homoplasy

incongruence

eucalypts

The utility of microsatellites (SSRs) in reconstructing phylogenies is largely confined to studies below the genuslevel, due to the potential of homoplasy resulting from allele size range constraints and poor SSR transferabilityamong divergent taxa. The eucalypt genus Corymbia has been shown to be monophyletic using morphological characters,however, analyses of intergenic spacer sequences have resulted in contradictory hypotheses- showing thegenus as either equivocal or paraphyletic. To assess SSR utility in higher order phylogeny in the family Myrtaceae,phylogenetic relationships of the bloodwood eucalypts Corymbia and related genera were investigated using eightpolymorphic SSRs. Repeat size variation using the average square and Nei's distance were congruent and showedCorymbia to be a monophyletic group, supporting morphological characters and a recent combination of the internaland external transcribed spacers dataset. SSRs are selectively neutral and provide data at multiple genomic regions,thus may explain why SSRs retained informative phylogenetic signals despite deep divergences. We show thatwhere the problems of size-range constraints, high mutation rates and size homoplasy are addressed, SSRs mightresolve problematic phylogenies of taxa that have diverged for as long as three million generations or 30 millionyears.

Details

Title: Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)
Authors: J W Ochieng (Author) - Southern Cross University
Dorothy A Steane (Author) - University of Tasmania
P Y Ladiges (Author) - University of Melbourne
P R Baverstock (Author) - Southern Cross University
R J Henry (Author) - Southern Cross University
M Shepherd (Author) - Southern Cross University
Publication details: Genetics and Molecular Biology, Vol.30(4), pp.1125-1134
Publisher: Sociedade Brasileira de Genetica
Date published: 2007
DOI: 10.1590/S1415-47572007000600016
ISSN: 1415-4757
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450297902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Genetics & Heredity